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首页> 外文期刊>African Journal of Biotechnology >Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1) gene
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Development of specific primers for genus Fusarium and F. solani using rDNA sub-unit and transcription elongation factor (TEF-1) gene

机译：利用rDNA亚基和转录延伸因子（TEF-1）基因开发镰刀菌和茄形镰刀菌特异性引物

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Detection of?Fusarium solani?causal agent of wilt and rots in many plant species are difficult if based only on morphological characteristics. Beside this,?morphological discrimination requires special skill and the expertise of taxonomists or specialists. To simplify the detection and discrimination of?F. solani?from other?Fusariumspecies, end-point polymerase chain reaction (PCR) assays were developed. Consensus sequences obtained from multiple alignments of?target genes, internal transcribed spacer (ITS), rDNA and transcription elongation factor (TEF-1α), were used to?design?the primers for rapid detection of genus?Fusarium?(amplified product 420 and 466) and?F. solani?(amplified product 658, 595 and 485).?BLASTn was used for?in silico?specificity. No cross reactivity was observed when primers were checked against the near-neighbor plant pathogens.?The described primer sets allowed accurate identification and discrimination of genus?Fusarium?and?F. solani.?All tests have multiple applications including screening of infected plants, breeding programs and disease diagnosis.
机译：如果仅基于形态特征，则很难检测许多植物中枯萎病和腐烂病的致病性。除此之外，形态学歧视还需要分类学家或专家的特殊技能和专业知识。为了简化对F的检测和区分。从其他ani类物种中获得了solani ?，开发了终点聚合酶链反应（PCR）分析方法。从靶基因，内部转录间隔区（ITS），rDNA和转录延伸因子（TEF-1α）的多重比对获得的共有序列被用于“设计”用于快速检测“ F”属的引物（扩增产物420和466）和？ solani？（扩增产物658、595和485）。BLASTn用于“计算机”特异性。检查引物是否针对邻近植物病原体时，未观察到交叉反应。所描述的引物组可准确鉴定和区分？属和F属。 solani。？所有测试都有多种应用，包括受感染植物的筛选，育种程序和疾病诊断。

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