...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Anti-Inflammatory molecular mechanisms of Bo-Ye-Niu-Pi-Xiao (Cynanchum taiwanianum Yamazaki)
【24h】

Anti-Inflammatory molecular mechanisms of Bo-Ye-Niu-Pi-Xiao (Cynanchum taiwanianum Yamazaki)

机译：anti-inflammatory molecular mechanisms of BO-Y厄-NI U-pi-ξ奥 (CY难处M台湾Ian um ya吗za开i)

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bo-Ye-Niu-Pi-Xiao,?Cynanchum taiwanianum?Yamazaki (family: Asclepiadaceae) is a well-known and popular herb; its rhizome has been used as a folk medicine in Taiwan. Many therapeutic effects of?C. taiwanianum?Yamaz. had been studied; however, there is still no anti-inflammatory effect and mechanism of?C. taiwanianumYamaz. reported. Besides, it is well-known that the phosphorylation of I kappa B-alpha (IκBα) to phospho-IκBα (p-IκBα) is a decisive step in the nuclear factor kappa B (NF-κB) activation pathway. In addition, the induction of cyclooxygenase-2 (COX-2) and inducible nitric oxide synthase (iNOS) by cytokines, such as interleukin-1β (IL-1β), is well known to be partly mediated by NF-κB. Hence, the objective of this study?was?to evaluate the activity and distinguish the mechanism of anti-inflammation of?C. taiwanianum?Yamaz. rhizome on rat kidney cell line NRK-52E induced by IL-1β. In the present study, we investigated the anti-inflammatory effect and potential mechanism of?C. taiwanianum?Yamaz. rhizome ethanolic extract (CTEE) in IL-1β-induced NRK-52E cells. Production of nitric oxide (NO) and prostaglandin E2 (PGE2) by enzyme linked immunosorbent assay. The messenger ribonucleic acid (mRNA) and protein expression of iNOS and COX-2, phosphorylation of IκBα, and activation of NF-κB was executed by reverse transcription-polymerase chain reaction and Western blotting. Results show that the CTEE significantly (P?< 0.05) inhibited NO and PGE2production and also significantly (P?< 0.05) attenuated protein and mRNA expression of iNOS and COX-2 in IL-1β-induced NRK-52E cells without obvious cytotoxic effects. Furthermore, the CTEE suppressed the NF-κB nuclear translocation, in terms of inhibition of IκBα phosphorylation. The results provided evidence for its folkloric uses and suggest that the anti-inflammatory activities of CTEE may result from the inhibition of inflammatory mediators, such as NO and PGE2?and an upstream suppression of NF-κB-dependent mechanism might be involved.
机译：Bo-Ye-Pi-Xiao，Cynanchum taiwanianum？Yamazaki（家族：Asclepiadaceae）是一种著名的流行草药。其根茎在台湾已被用作民间药物。 C的许多治疗作用。 taiwanianum？Yamaz。经过研究;但是，仍没有消炎作用和消炎药的作用机理。 taiwanianumYamaz。报告。此外，众所周知，IκB-α（IκBα）磷酸化为磷酸-IκBα（p-IκBα）是核因子κB（NF-κB）激活途径的决定性步骤。另外，众所周知，白介素-1β（IL-1β）等细胞因子对环氧合酶2（COX-2）和可诱导型一氧化氮合酶（iNOS）的诱导部分地由NF-κB介导。因此，本研究的目的是评估活性并区分C的抗炎机制。 taiwanianum？Yamaz。 IL-1β诱导的根茎对大鼠肾细胞系NRK-52E的影响。在本研究中，我们研究了？C的抗炎作用及其潜在机制。 taiwanianum？Yamaz。 IL-1β诱导的NRK-52E细胞中的根茎乙醇提取物（CTEE）。通过酶联免疫吸附测定法生成一氧化氮（NO）和前列腺素E2（PGE2）。通过逆转录-聚合酶链反应和蛋白质印迹法进行信使核糖核酸（mRNA）和iNOS和COX-2的蛋白表达，IκBα的磷酸化以及NF-κB的激活。结果显示，CTEE显着（P <0.05）抑制NO和PGE2的产生，并且显着（P <0.05）减弱IL-1β诱导的NRK-52E细胞中iNOS和COX-2的蛋白质和mRNA表达，而没有明显的细胞毒性作用。此外，就抑制IκBα磷酸化而言，CTEE抑制了NF-κB核易位。该结果提供了其民用用途的证据，并表明CTEE的抗炎活性可能是由炎性介质（如NO和PGE2？）的抑制引起的，并且可能涉及上游抑制NF-κB依赖性机制。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号