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The influence of biopsy method on the survival rates of sexed and cryopreserved bovine embryos

机译:活检方法对有性和低温保存的牛胚胎存活率的影响

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The purpose of this research was to find the most suitable embryo biopsy technique that would yield the highest pregnancy rates after the transfer of sexed frozen-thawed bovine embryos. Three methods of embryo biopsy (needle, blade or aspiration) were applied on 120 bovine embryos divided into 3 batches (n = 40). Subsequently, the embryos were frozen/thawed and transferred to synchronized recipients. Pregnancy diagnosis was performed 30 days later using an ultrasound scanner. The results show a significant difference in pregnancy rates according to the biopsy method: 55% for the needle biopsy, 45% for the aspiration method and 30% for the microblade technique.
机译:这项研究的目的是找到最合适的胚胎活检技术,该技术将在性交冻融的牛胚胎移植后产生最高的妊娠率。将三种活检方法(针,刀片或抽吸法)应用于120头分为三批(n = 40)的牛胚胎。随后,将胚胎冷冻/解冻并转移至同步的接受者。 30天后使用超声扫描仪进行妊娠诊断。结果显示,根据活检方法,妊娠率有显着差异:针刺活检的比率为55%,抽吸法为45%,微刀片技术为30%。

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