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首页> 外文期刊>African Journal of Biotechnology >Genetic diversity and identification of variety-specific AFLP markers in fenugreek (Trigonella foenum-graecum)
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Genetic diversity and identification of variety-specific AFLP markers in fenugreek (Trigonella foenum-graecum)

机译：胡芦巴（Trigonella foenum-graecum）的遗传多样性和品种特异性AFLP标记的鉴定

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Fenugreek (Trigonella foenum-graecum),?an important spice crop belongs to the family Fabaceae. India is the largest producers as well as the largest exporter of fenugreek. Five common fenugreek varieties of India were included in this study for molecular analysis. For randomly amplified polymorphic DNA (RAPD) analysis, a total of 47 bands in the size range of 200 to 5000 bp were produced by examining across genotypes with 9 RAPD primers, with an average polymorphism of 62.4%. Total numbers of bands ranged from 3 (U407) to 7 (OPB-15). With 17 fluorescently labeled amplified fragment length polymorphism (AFLP) primer combinations (PCs), a total of 669 peaks in the size range of 50 to 538 bp were amplified. Total numbers of bands ranged from 21 (E-AAC/M-CT) to 60 (E-AAC/M-CAC). The mean genetic diversity (Nei's 1973) across all loci was found?to be?23.83 and 2.1% with RAPD and AFLP markers, respectively. In all the fenugreek varieties, a?total of 25 variety-specific AFLP markers were found. Phylogenetic trees among 5 plant varieties were constructed based on Nei's coefficient standard genetic distances using unweighted pair groupmethod?with arithmetic mean (UPGMA) method. For RAPD and AFLP analysis, Gujarat Methi-1 (GM-1) and Gujarat Methi-2 (GM-2) clustered together showed more similarities than other varieties. In fenugreek, RAPD markers were found more polymorphic than AFLP markers. RAPD markers also showed more diversity in comparison to AFLP markers, although the data generated for AFLP markers were more authenticated and reproducible than RAPD markers.
机译：胡芦巴（Trigonella foenum-graecum），一种重要的香料作物，属于豆科（Fabaceae）。印度是胡芦巴的最大生产国和最大出口国。本研究包括印度的五个胡芦巴常见变种进行分子分析。对于随机扩增多态性DNA（RAPD）分析，通过使用9种RAPD引物检查基因型，共产生了200到5000 bp大小的47条带，平均多态性为62.4％。频段总数从3（U407）到7（OPB-15）不等。用17种荧光标记的扩增片段长度多态性（AFLP）引物组合（PC），可扩增出大小范围为50到538 bp的669个峰。频段总数从21（E-AAC / M-CT）到60（E-AAC / M-CAC）不等。发现所有基因座的平均遗传多样性（Nei 1973）用RAPD和AFLP标记分别为23.83和2.1％。在所有胡芦巴变种中，总共发现了25种变种特异性AFLP标记。根据Nei系数标准遗传距离，采用算术平均（UPGMA）非加权对群法构建了5个植物品种的系统树。对于RAPD和AFLP分析，聚在一起的古吉拉特邦Methi-1（GM-1）和古吉拉特邦Methi-2（GM-2）表现出比其他品种更多的相似性。在胡芦巴中，发现RAPD标记比AFLP标记具有更多的多态性。与AFLP标记相比，RAPD标记还显示出更多的多样性，尽管为AFLP标记生成的数据比RAPD标记更可靠和可重复。

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