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首页> 外文期刊>African Journal of Biotechnology >Protective effect and expression of defense-related ESTs induced by acibenzolar-S-methyl and a phosphorylated mannan oligosaccharide-based product against Moniliophthora perniciosa in Theobroma cacao
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Protective effect and expression of defense-related ESTs induced by acibenzolar-S-methyl and a phosphorylated mannan oligosaccharide-based product against Moniliophthora perniciosa in Theobroma cacao

机译：苯甲酰-S-甲基酯和磷酸化的甘露寡糖基产物诱导的可乐虫对百日咳单孢菌的保护作用及防御相关EST的表达

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摘要

Witches’ broom disease (WBD), caused by the fungus?Moniliophthora perniciosa, is one of the main diseases in cocoa (Theobroma cacao)?and has caused severe economic losses. Integrated disease management has been the focus for its control and therefore, the identification of new inducers of plant resistance is desirable. Thus, the goal of this work was to evaluate two potential inducers of resistance against WBD. A phosphorylated mannan oligosaccharide-based product (PMO) and acibenzolar S-methyl (ASM) were tested on?M. perniciosa?inoculated seedlings and in field experiments and showed a reduction on the incidence of WBD. The expression of two defense-related?expressed sequence tags (ESTs)?in cocoa, coding for peroxidase (Pox) and chitinase (Chi), were accessed by qPCR. Both products induced the expression of the?Pox?defense-related EST. In general, ASM induced the expression of chitinase (Chi)?and peroxidase?(Pox) in earlier time-points than PMO. However, PMO provided long-lasting and higher levels of expression.?Chi?expression was triggered in the time-points succeeding the spraying but was very low. On the other hand, peaks of?Pox?transcripts were detected in later time-points for both inducers. ASM and PMO modes of action might be explained, at least partially, by the overexpression of defense-related ESTs.
机译：女巫的扫帚病（WBD）是由真菌Moniliophthora perniciosa引起的，是可可（Theobroma cacao）的主要疾病之一，已造成严重的经济损失。病害综合防治一直是其控制的重点，因此，鉴定新的植物抗性诱导剂是可取的。因此，这项工作的目的是评估两种抗WBD的潜在诱导剂。在？M上测试了磷酸化的甘露寡糖基产品（PMO）和苯并苄基S-甲基（ASM）。多年生植物接种了多年生植物;在野外实验中，显示出WBD发生率的降低。通过qPCR获得了可可中两个与防御相关的表达序列标签（ESTs）的表达，分别编码过氧化物酶（Pox）和几丁质酶（Chi）。两种产物均诱导了与Pox防御相关的EST的表达。通常，ASM在比PMO更早的时间点诱导几丁质酶（Chi）？和过氧化物酶（Pox）的表达。但是，PMO提供了持久且更高水平的表达。在喷施后的时间点触发了Chi-表达，但表达非常低。另一方面，在两个诱导物的较晚时间点都检测到了Poxβ转录物的峰。 ASM和PMO的行动模式至少可以通过与国防相关的EST的过度表达来解释。

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