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Characterization of Quercus species distributed in Jordan using morphological and molecular markers

机译:利用形态学和分子标记物表征约旦分布的栎属物种

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Genetic diversity among 25 natural populations of three different species of?Quercusin Jordan at morphological and molecular levels using random amplified polymorphic DNA (RAPD) primers was assessed. Significant morphological and molecular variations among and within 25?Quercus?populations were estimated. Standardized canonical discrimination functions for the investigated morphological traits showed that the first function explains 72.44% of the total variability between populations and was strongly influenced by leaf and scale length. Twenty-seven polymorphic markers and 5917 scored bands were generated using six RAPD?primers. Based on morphological and RAPD data, the populations were grouped together in the same cluster according to species regardless of local of collections. Result of cluster analysis based on RAPD showed significant correlation with morphological characters based on Mantel’s test (r = 0.145**). This study has emphasized the ability of the morphological and molecular markers in determining the genetic diversity among and within the populations of?Quercus?and that the resulted high genetic variability could be utilized in implications of improving conservation, restoration and reforestation strategies of?Quercus?in Jordan.
机译:使用随机扩增多态性DNA(RAPD)引物评估了形态学和分子水平上三种不同种类的Quercusin Jordan的25个自然种群的遗传多样性。估计25个栎属种群之间和之内的重要形态和分子变异。针对所调查形态特征的标准化规范判别函数表明,第一个函数解释了种群之间总变异的72.44%,并且受到叶和鳞片长度的强烈影响。使用六个RAPD?引物产生了27个多态性标记和5917得分带。根据形态和RAPD数据,无论物种的收集地点如何,都可以根据物种将种群归为同一类。基于RAPD的聚类分析结果与基于Mantel检验的形态特征具有显着相关性(r = 0.145 **)。这项研究强调了形态学和分子标记物在确定栎种群之间及其内部遗传多样性方面的能力,并且所产生的高遗传变异性可用于改善栎的保护,恢复和重新造林策略。在约旦。

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