• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Cryopreservation of in vitro-grown shoot tips of apricot (Prunus armeniaca L.) using encapsulation-dehydration
【24h】

Cryopreservation of in vitro-grown shoot tips of apricot (Prunus armeniaca L.) using encapsulation-dehydration

机译：包囊脱水冷冻保存杏（Prunus armeniaca L.）的离体芽尖端

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In vitro?grown apricot (Prunus armeniaca?L.) cv.?El-Hamawey shoot tips were successfully cryopreserved using an encapsulation-dehydration procedure. Shoot tips were encapsulated in calcium-alginate beads before preculture on woody plant (WP) medium supplemented with different sucrose concentrations; 0.1, 0.3, 0.5, 0.75 and 1.0 M for 2, 4, 6 and 8 days at 25°C. Encapsulated shoot tips were then dehydrated by incubation in the sterile air flow of a laminar air-flow cabinet for 0.0 - 6 h and immediately plunged into liquid nitrogen (LN, -196°C). The highest survival (100%) and regrowth (92.3%) rates were obtained with encapsulated unfrozen apricot shoot tips that were precultured on WP medium containing 0.5 M sucrose for two days and followed by dehydration for 2 h. After recovering from liquid nitrogen and rapid thawing in a water bath at 38°C for 2 to 3 min, the greatest survival (74.5%) and regrowth (71.8%) were obtained and encapsulated shoot tips were precultured for two days in WP medium containing 0.75 M sucrose dehydrated for 2 h, desiccated to 19.55% moisture content and conserved three months in liquid nitrogen. After two years in liquid nitrogen storage,?the greatest regrowth percentage (60.4%) was obtained when encapsulated cryopreserved shoot tips were precultured for two days in medium containing 0.75 M sucrose and also with 2 h of dehydration.?This study has successfully developed a simple and effective protocol for the cryopreservation of apricot shoot tips.?Genetic stability of?plantlets derived from cryopreserved shoot tips was evaluated using random amplification of polymorphic DNA (RAPD) markers.
机译：使用封装-脱水程序成功冷冻保存了体外生长的杏（李子）v。？-Hamawey茎尖。将芽尖封装在藻酸钙珠粒中，然后在补充了不同蔗糖浓度的木本植物（WP）培养基上进行预培养; 0.1、0.3、0.5、0.75和1.0 M在25°C下放置2、4、6和8天。然后，通过在层流气流柜的无菌气流中孵育0.0-6小时，使封装的芽尖脱水，然后立即将其浸入液氮（LN，-196°C）中。用封装的未冷冻杏芽梢获得最高的存活率（100％）和再生长率（92.3％），将其在含0.5M蔗糖的WP培养基上预培养两天，然后脱水2小时。从液氮中回收并在38°C的水浴中快速融化2至3分钟后，获得最大的存活率（74.5％）和再生长（71.8％），并将封装的芽尖在含有WP的WP培养基中预培养两天0.75 M蔗糖脱水2小时，干燥至水分含量为19.55％，并在液氮中保存三个月。在液氮中储存两年后，将封装的冷冻保存的芽尖在含有0.75 M蔗糖的培养基中脱水2 h进行预培养两天，可获得最大的再生长百分比（60.4％）。冷冻保存杏枝梢的简单有效方法。使用多态性DNA（RAPD）标记随机扩增，评估了冷冻保存枝梢的小植株的遗传稳定性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号