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An active form of calcium and calmodulin dependant protein kinase (ccamk) of Medicago truncatula

机译:ca藜苜蓿钙和钙调蛋白依赖性蛋白激酶(ccamk)的活性形式

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The DMI3 gene of the model legume Medicago truncatula encodes a calcium and calmodulin dependent protein kinase (CCaMK) involved in the signalling pathways leading to the establishment of both mycorrhizal and rhizobial root symbiosis. The removal of the auto-inhibitory domain that negatively regulates the kinase activity in M. truncatula results in a constitutively-active form, inducing symbiotic responses in the absence of bacterial signals. In this study, we verified the functionality of a DMI3 variant and its ability to induce spontaneous nodules in M. truncatula dmi3 mutant. Our results based on enzymatic radio activity assay using [g-33P] ATP, suggests that the DMI3-311 variant is active and its corresponding gene (DMI3-311) when introduced in the dmi3 mutant, by Agrobacterium rhizogenes transformation, induced in the formation of a few spontaneous nodules.
机译:豆科植物苜蓿模型的DMI3基因编码钙和钙调蛋白依赖性蛋白激酶(CCaMK),参与导致菌根和根瘤菌共生的信号传导途径。消极调节截形支原体中激酶活性的自抑制域的去除导致组成型活性形式,在没有细菌信号的情况下诱导共生反应。在这项研究中,我们验证了DMI3变体的功能及其在truncatula dmi3突变体中诱导自发结节的能力。我们基于使用[g-33P] ATP的酶活性测定的结果表明,通过发根农杆菌转化,将DMI3-311变体激活,并将其相应基因(DMI3-311)引入dmi3突变体中,一些自发的结节。

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