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首页> 外文期刊>African Journal of Biotechnology >Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA
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Micropropagation of Guadua angustifolia Kunth (Poaceae) using a temporary immersion system RITA

机译：使用临时浸没系统RITA对瓜杜鹃（禾本科）进行微繁殖

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The micropropagation of Guadua angustifolia Kunth, commonly known as giant bamboo, using semi-solid culture medium, is reported to have low multiplication rates. This study evaluated the multiplication index of G. angustifolia in a temporary immersion system (RITA?), comparing results with those obtained using a semi-solid culture medium. The treatments consisted of either three or four 2-min immersions per day and use of semi-solid culture medium, which consisted of MS supplemented with 3.0 mg L-1 of the cytokinin benzylaminopurine (BAP). Equipment consisted of 20 vessels for automated RITA?, each containing 200 ml of culture medium. Immersions were performed for 2 min at two different frequency intervals (6 and 8 h). Large clumps of G. angustifolia with 1, 2 or 3 stems were inoculated depending on the treatment. Best results were obtained with four immersion cycles per day (every 6 h), with a multiplication index of 2.7 shoots per original explant (axillary buds) and greater rhizome growth. Overall, the temporary immersion system performed better than the semi-solid medium in terms of shoot multiplication rates and rhizome growth. Further studies should be conducted to develop an application for RITA? for use in the commercial production of G. angustifolia.
机译：据报道，使用半固体培养基对小鳞茎瓜（Guadua angustifolia Kunth）进行微繁殖，繁殖率很低。这项研究评估了在临时浸没系统（RITA？）中的古氏茶的繁殖指数，并将结果与​​使用半固体培养基获得的结果进行了比较。处理包括每天浸泡3次或4次2分钟，并使用半固体培养基，该培养基由MS补充3.0 mg L-1的细胞分裂素苄氨基嘌呤（BAP）组成。设备由20个用于自动RITA？的容器组成，每个容器包含200 ml培养基。以两个不同的频率间隔（6和8小时）浸入2分钟。根据处理情况，接种了大块的带有1、2或3个茎的G. angustifolia。每天四个浸入周期（每6小时）获得最佳结果，每个原始外植体（腋芽）的繁殖指数为2.7枝，根茎生长更大。总体而言，临时浸没系统在枝条繁殖率和根茎生长方面表现优于半固态培养基。应进行进一步研究以开发RITA应用程序？用于商业生产的G. angustifolia。

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