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首页> 外文期刊>African Journal of Biotechnology >Drought tolerance in transgenic tropical maize (Zea mays L.) by heterologous expression of peroxiredoxin2 gene-XvPrx2
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Drought tolerance in transgenic tropical maize (Zea mays L.) by heterologous expression of peroxiredoxin2 gene-XvPrx2

机译：Peroxiredoxin2基因-XvPrx2的异源表达对转基因热带玉米（Zea mays L.）的耐旱性

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摘要

Transformation of a tropical maize inbred genotype (CML144) with the Xerophyta viscosa peroxiredoxin2 (XvPrx2) gene was reported. The protective role of peroxiredoxin2 against the damage resulting from reactive oxygen species (ROS) under dehydration stress was further determined. Successful integration of XvPrx2 gene into maize we achieved and recovered 10 independent transgenic events. Transformation and regeneration frequencies were 12.9 and 31.3%, respectively. Reverse transcription polymerase chain reaction (PCR) revealed the expression of the XvPrx2 gene in transformed plants under dehydration. Stressed transgenic plants had higher relative water content (RWC) as compared to the conventional plants. Recovery irrigation showed higher RWC in transgenics than in conventional plants. Unlike in conventional plants, rapid morphogenic recovery was observed in transgenics within 24 h. Chlorophyll contents decreased faster in conventional plants than in transgenics with prolonged drought. Generally, transgenic plants were more tolerant to dehydration stress than conventional plants. This tolerance may be associated with the over expression of peroxiredoxin2 playing a role in managing ROS generated in plant cells.
机译：据报道，热带干玉米自交基因型（XML）转化了Xerophyta viscosa peroxiredoxin2（XvPrx2）基因。进一步确定了peroxiredoxin2在脱水胁迫下对活性氧（ROS）造成的损害的保护作用。 XvPrx2基因成功整合到玉米中，我们实现并恢复了10个独立的转基因事件。转化和再生频率分别为12.9％和31.3％。逆转录聚合酶链反应（PCR）显示XvPrx2基因在脱水条件下在转化植物中的表达。与常规植物相比，胁迫的转基因植物具有较高的相对含水量（RWC）。恢复灌溉显示转基因植物中的RWC高于常规植物。与常规植物不同，转基因植物在24小时内观察到快速的形态发生恢复。与长期干旱的转基因植物相比，常规植物的叶绿素含量下降得更快。通常，转基因植物比常规植物更耐脱水胁迫。这种耐受性可能与过氧化物酶2的过表达有关，过氧化物酶2在管理植物细胞中产生的ROS中起作用。

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