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首页> 外文期刊>African Journal of Biotechnology >Trichoderma atroviride 102C1: A promising mutant strain for the production of a -glucosidase, ?-xylosidase and ?-L-arabinofuranosidase activities using agroindustrial by-products
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Trichoderma atroviride 102C1: A promising mutant strain for the production of a -glucosidase, ?-xylosidase and ?-L-arabinofuranosidase activities using agroindustrial by-products

机译：木霉atroviride 102C1：一种有前途的突变菌株，可利用农用工业副产物生产α-葡萄糖苷酶，β-木糖苷酶和β-L-阿拉伯呋喃糖苷酶活性

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Holocellulolytic accessory enzymes are very important in assisting hydrolysis of biomass. The use of these enzymes in the enzymatic hydrolysis of plant biomass is very important for obtaining building blocks in the concept of biorefinery. In previous studies, the mutant strain Trichoderma atroviride 102C1 was tested for production of endoglucanases, FPases and endoxylanases. This study aimed at evaluating the efficiency in holocellulolytic accessories enzymes production (b-glucosidase, b-xylosidase and a-L-arabinofuranosidase) by Trichoderma atroviride 102C1 using different lignocelluloses biomass as substrates. Accessory enzymes production was carried out in Erlenmeyer flasks containing Mandels salt medium, supplemented with different concentrations of sugarcane bagasse (SCB) and corn steep liquor (CSL), according to a Central Composite Rotational Design (CCRD). The fermentation system was incubated under agitation for 2 days / 28°C. For pH and temperature profile studies, a new CCRD was carried out. The best condition common to all enzymes, 55.4 U.mL-1 (β-glucosidase), 10.8 U.mL-1 (β-xylosidase) and 143.23 U.mL-1 (α-L-arabinofuranosidase), was observed when 2.5% (w/v) sugarcane bagasse (SCB) and 1.26% (w/v) of corn steep liquor (CSL) were used. All enzymes presented acidophilic characteristic in two different temperatures (44 and 55°C). The optimal profile characteristic for β-glucosidase and β-xylosidase activities were pH 5.0 and 3.0, respectively, both at 55°C, while for α-L-arabinofuranosidase it was pH 3.6 at 44°C. This study demonstrated the potential of T. atroviride 102C1 to produce three important holocellulolytic accessory enzymes in the presence of SCB and CSL, suggesting its use for enzymatic hydrolysis of lignocellulosic biomass.
机译：纤维素分解辅助酶在协助生物质水解中非常重要。这些酶在植物生物质的酶促水解中的使用对于获得生物精炼概念中的基本组成部分非常重要。在以前的研究中，测试了突变菌株阿斯特木霉木霉102C1的内切葡聚糖酶，FPase和内切木聚糖酶的产生。这项研究的目的是评估木霉阿troviride 102C1使用不同的木质纤维素生物质作为底物来生产全纤维素分解辅助酶（b-葡萄糖苷酶，b-木糖苷酶和a-L-阿拉伯呋喃糖苷酶）的效率。根据中央复合旋转设计（CCRD），在含有曼德尔盐培养基的锥形瓶中进行辅酶的生产，该烧瓶中添加了不同浓度的甘蔗渣（SCB）和玉米浆（CSL）。在搅拌下将发酵系统孵育28天/ 2天。为了研究pH和温度曲线，进行了新的CCRD。当2.5时，观察到所有酶共有的最佳条件是55.4 U.mL-1（β-葡萄糖苷酶），10.8 U.mL-1（β-木糖苷酶）和143.23 U.mL-1（α-L-阿拉伯呋喃糖苷酶）使用％（w / v）的甘蔗渣（SCB）和1.26％（w / v）的玉米浆（CSL）。所有的酶在两个不同的温度（44和55°C）下均表现出嗜酸特性。 β-葡萄糖苷酶和β-木糖苷酶活性的最佳特征曲线分别在55°C下为pH 5.0和3.0，而对于α-L-阿拉伯呋喃糖苷酶，在44°C下为pH 3.6。这项研究表明，在存在SCB和CSL的情况下，T。atroviride 102C1可能产生三种重要的全纤维素分解辅助酶，表明其可用于木质纤维素生物质的酶促水解。

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