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首页> 外文期刊>African Journal of Biotechnology >New laccase-producing fungi isolates with biotechnological potential in dye decolorization
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New laccase-producing fungi isolates with biotechnological potential in dye decolorization

机译：新型的产生漆酶的真菌分离物具有染料脱色的生物技术潜力

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Thirty six?(36)?native fungal strains from the state of Yucatan were first screened for ligninolytic activity on solid media containing ABTS.?Molecular identification based on ITS rDNA region and PCR fingerprinting of seven selected fungi isolates?werecarried out. Molecular characterization based on genetic fingerprinting was helpful in determining unequivocally the differences between isolates at genera and species levels. The seven isolates showed ABTS oxidation?zones in plates but only five strains produced extracellular laccase. The strains identified as?Trametes?hirsuta(GenBank accession numbers GQ280372 and GQ280373)?showed the highest laccase production. The strain?Bm-2?displayed the greatest laccase activity and dye decolourization ability in 72 h without the addition of mediators. Both the high laccase activity shown by Bm-2 and its ability to decolorize dyes are a good indication of its possible use in the treatment of textile effluents.
机译：首先筛选了来自尤卡坦州的36株（36）原生真菌菌株在含有ABTS的固体培养基上的木质素分解活性。进行了基于ITS rDNA区域的分子鉴定和7种选定真菌的PCR指纹图谱。基于遗传指纹的分子表征有助于明确确定分离株在属和种水平上的差异。七个分离株在平板中显示ABTS氧化区，但只有五个菌株产生细胞外漆酶。鉴定为“ Trametes” hirsuta（GenBank登录号GQ280372和GQ280373）的菌株显示出最高的漆酶产量。在没有添加介体的情况下，菌株“ Bm-2”在72小时内表现出最大的漆酶活性和染料脱色能力。 Bm-2所显示的高漆酶活性及其使染料脱色的能力都很好地表明了其可能用于处理纺织废水。

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