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首页> 外文期刊>African Journal of Biotechnology >Molecular assessment of clarithromycin resistant Helicobacter pylori strains using rapid and accurate PCR-RFLP method in gastric specimens in Iran
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Molecular assessment of clarithromycin resistant Helicobacter pylori strains using rapid and accurate PCR-RFLP method in gastric specimens in Iran

机译：快速准确的PCR-RFLP方法在伊朗胃标本中对克拉霉素耐药的幽门螺杆菌菌株进行分子评估

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摘要

Currently, a seven-day, triple-drug regimen has been recommended as one of the first-line therapies for?Helicobacter pylori?management in which clarithromycin is a key component. Development of clarithromycin resistance leads to the long term assessment of the efficacy of clarithromycin in the triple-drug regimen. The aim of this?study was to rapidly and directly assess clarithromycin resistance point mutations on gastric biopsy specimens by using PCR-RFLP method. Biopsy samples were obtained over a 6-months period of 2009, from 200 dyspeptic patients referred to Shahrekord University of Medical Sciences, Iran. Initially, rapid urease test was performed and then DNA was isolated from each tissue and used for molecular analysis such as PCR (for?H. pylori?diagnostic) and PCR-RFLP (for Cla resistance determination). RUT and PCR results showed that 164 (82%) of the patients were?H. pylori-positive. Resistance was evaluated in 164 samples by using enzymes?BsaI?and?MboII. Thirty nine?(39)?(23/78%) clarithromycin-resistant strains were detected which were identified as 15 (9.15%) A2143G, 15 (9.15%) A2142G and 9 (5.49%) mix strains. The results showed that PCR-RFLP method had?a high accuracy to detect A2142G and A2143G mutations associated with resistance to clarithromycin in the minimum possible time.
机译：目前，推荐使用7天三联疗法作为幽门螺杆菌管理的一线治疗方法之一，其中克拉霉素是关键成分。克拉霉素耐药性的发展导致对克拉霉素在三联疗法中的疗效进行长期评估。这项研究的目的是使用PCR-RFLP方法快速，直接地评估胃活检标本上的克拉霉素抗性点突变。 2009年的6个月期间，从伊朗Shahrekord医学大学的200名消化不良患者获得了活检样本。最初，进行了快速尿素酶测试，然后从每个组织中分离出DNA，并用于分子分析，例如PCR（用于幽门螺杆菌诊断）和PCR-RFLP（用于Cla抗性测定）。 RUT和PCR结果显示164名患者（82％）为？H。幽门螺杆菌阳性。使用酶BsaI和MboII评估了164个样品的抗性。检出三十九（39）（23/78％）克拉霉素抗性菌株，鉴定为15（9.15％）A2143G，15（9.15％）A2142G和9（5.49％）混合菌株。结果表明，PCR-RFLP方法在尽可能短的时间内检测与克拉霉素耐药相关的A2142G和A2143G突变具有很高的准确性。

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