• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Genetic manipulation of fungal strains for the improvement of heterologous genes expression (a mini-review)
【24h】

Genetic manipulation of fungal strains for the improvement of heterologous genes expression (a mini-review)

机译：真菌菌株的遗传操作可改善异源基因的表达（综述）

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Fungi are eukaryotic microorganisms that influence our everyday lives in areas as diverse as agriculture, medicine and basic science.?With the advent of molecular biology, it has been attempted to improve the expression level of heterologous genes, which encode favorable traits in fungal strains.?The expression of heterologous genes can be limited in transcription, post-transcription, translation and post-translation levels. Several genetic strategies have been developed to reduce the expression constrains?and to enhance genes functionality. Among these strategies can be pointed to the introduction of multicopies of the desired gene, change of AT-rich sequences, gene fusion with a well-expressed gene, the use of strong promoters and signal sequences, optimization of codon usage, the construction and use of protease-deficient and chaperones/foldases-overproduced? strains and the use of native or artificial intron-containing genes. These strategies have often resulted in the expected increase in the expression of heterologus genes.? With the isolation of a large number of genes encoding desired traits and the availability of a large collection of wild isolates, the improvement of strains with a better functional performance would be possible.
机译：真菌是真核微生物，影响农业，医学和基础科学等领域的日常生活。随着分子生物学的出现，人们尝试提高异源基因的表达水平，该基因编码真菌菌株中的有利特性。异源基因的表达可以在转录，转录后，翻译和翻译后水平上受到限制。已经开发了几种遗传策略来减少表达限制并增强基因功能。在这些策略中，可以指出所需基因的多拷贝的引入，富AT序列的改变，与表达良好的基因的基因融合，强启动子和信号序列的使用，密码子使用的优化，构建和使用。缺乏蛋白酶和伴侣/折叠酶的过度生产？菌株和含有天然或人工内含子的基因的使用。这些策略通常导致异源基因表达的预期增加。随着大量编码期望性状的基因的分离以及大量野生分离株的可用性，具有更好功能性能的菌株的改良将是可能的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号