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首页> 外文期刊>African Journal of Biotechnology >In vitro assessment of bioactive components of Mirabilis jalapa ethanolic extract on clinical isolates of Salmonella typhi and Bacillus cereus
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In vitro assessment of bioactive components of Mirabilis jalapa ethanolic extract on clinical isolates of Salmonella typhi and Bacillus cereus

机译：紫茉莉乙醇提取物对伤寒沙门氏菌和蜡状芽孢杆菌临床分离株生物活性成分的体外评估

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We investigated the effects of bioactive components of ethanolic leaf extract ofMirabilis jalapa?on the disease causing enteric pathogens by testing their activity against?Salmonella typhi?and?Bacillus cereus?at an initial stock solution of 20 mg/ml of the crude extract. Following serial dilutions of stock solution, and the determination of minimum inhibitory concentration (MIC) and?minimum bactericidal concentration (MBC) of the crude extract, bioautography technique was used to identify bioactive fractions present in the crude extract. The observed fraction active against both organisms was separated and the MIC and MBC determined. Zones of inhibition for the crude extract at 20 ug/L were 34.33 mm±1.70 and 51.33 mm±1.88 for?S. typhi?and?B. cereus,?respectively while the zones of inhibition of separated bioactive fraction at 3mg/ml were 40.33 mm±1.33 and 40.67 mm±1.70 respectively. The MIC and MBC of the bioactive fraction showed increased in efficacy against the organisms as compared to that of the crude extract.?Fourier transform infrared spectroscopy (FTIR) and gas chromatography mass spectrometry (GC-MS) analyses of the bioactive fraction were carried out on the analyte under investigation to identify the constituents of the separated bioactive fraction. The results suggest that the antibacterial activity of the plant extract may be attributed to the alkaloids present.
机译：我们通过在20 mg / ml粗提液的初始储备液中测试其对鼠伤寒沙门氏菌和蜡状芽孢杆菌的活性，研究了紫茉莉乙醇叶提取物的生物活性成分对引起肠病原体的疾病的影响。连续稀释原液，并确定粗提取物的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）之后，使用生物自显影技术鉴定粗提取物中存在的生物活性成分。分离出观察到的对两种生物都有活性的部分，并确定MIC和MBC。粗提物在20 ug / L时的抑制作用范围为34.33 mm±1.70和51.33 mm±1.88。伤寒？在3mg / ml时，分离的生物活性级分的抑制区域分别为40.33mm±1.33和40.67mm±1.70。与粗提物相比，该生物活性部分的MIC和MBC表现出对微生物的功效增强。进行了该生物活性部分的傅里叶变换红外光谱（FTIR）和气相色谱质谱（GC-MS）分析对正在研究的分析物进行鉴定，以鉴定分离出的生物活性级分的成分。结果表明，植物提取物的抗菌活性可能归因于存在的生物碱。

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