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首页> 外文期刊>African Journal of Biotechnology >Correlation between salt tolerance and genetic diversity between Sulla carnosa and Sulla coronaria
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Correlation between salt tolerance and genetic diversity between Sulla carnosa and Sulla coronaria

机译：食盐沙雷和冠状沙雷之间耐盐性与遗传多样性的相关性

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摘要

Sulla?constitutes an important genetic resource contributing to pastoral production, particularly in semi-arid regions. In Tunisia, seedlings of the southern species?Sulla carnosa?(Desf.) and?the northern species?Sulla coronaria?(L.) were treated with NaCl (0, 100 and 200 mM) for 27 days. Salt treatments decreased leaf dry matter more in?S. carnosa?than in?S. coronaria.?S. coronaria?accumulated less Na+?and greater amounts of K+?and showed greater K/Na selectivity, a trait which could be related to the maintenance of higher net K+?uptake and transport in the presence of NaCl. Pigments were severely affected by salt stress in leaves of?S. carnosa?when compared with leaves of?S. coronaria. In addition to these physiological characterisations, genetic diversity was measured between the two accessions using inter simple sequence repeat (ISSR) markers. Three ISSR primers generated a total of 63 DNA amplicons for?S. carnosa?and 64 DNA amplicons for?S. coronaria, all of which were polymorphic between the two accessions. Correlations between the molecular and physiological data revealed statistically significant correlations between the salt response of these two?Sulla?accessions and two molecular markers B340 and B860, in roots and shoots, respectively.?S. coronaria?showed greater salt tolerance on the basis of growth and K/Na selectivity, making it a good candidate for inclusion in a future breeding programme.
机译：沙勒菌构成了重要的遗传资源，对牧业的生产尤其是半干旱地区的牧业作出了贡献。在突尼斯，用NaCl（0、100和200 mM）处理了南部物种Carnosa？（Desf。）和北部物种Sulla coronaria？（L。）的幼苗27天。盐处理可以使叶片中的干物质减少更多。卡诺萨（Carnosa）？科罗纳里亚。冠状菌积累的Na +较少，而K +的含量较高，并且具有较高的K / Na选择性，这可能与在NaCl存在下维持较高的净K +吸收和转运有关。盐胁迫严重影响了叶片的色素。 Carnosa？与S？的叶子相比。科罗纳里亚。除了这些生理特征外，还使用内部简单序列重复（ISSR）标记对两个种之间的遗传多样性进行了测量。三种ISSR引物共产生63个用于？S的DNA扩增子。 Carnosa？和64个DNA扩增子用于S。冠状花序，在两个种之间都是多态的。分子和生理数据之间的相关性揭示了这两个“ Sulla”种质的盐分响应与两个分子标记B340和B860分别在根和芽中的统计学显着相关性。在生长和钾/钠选择性的基础上，coronaria®表现出更高的耐盐性，使其成为未来育种计划的良好候选者。

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