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首页> 外文期刊>African Journal of Biotechnology >Molecular analysis of quality protein (QPM) and normal maize varieties from the DR-Congo breeding program
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Molecular analysis of quality protein (QPM) and normal maize varieties from the DR-Congo breeding program

机译：DR-刚果育种计划的优质蛋白质（QPM）和正常玉米品种的分子分析

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In recent years, the use of quality protein maize (QPM) with the objective of improving the nutritional value of maize grain protein has been the focus of several African breeding programs. Knowledge of genetic variation among QPM and normal maize varieties is important for an efficient selection and development of new varieties.The main objective of this study was to assess the level of genetic variation and relatedness among and within QPM and normal maize varieties selected in the DR-Congo breeding program using inter-simple sequence repeat (ISSR) and?random amplified polymorphic DNA?(RAPD) markers. For ISSR analysis, the mean level of polymorphism within each accession analyzed was 69%. Nei’s gene diversity (h) was 0.26 on the average. The genetic distance among the accessions varied from 0.098 to 0.460. More than 80% of genetic distances were below 0.35. The mean level of polymorphic loci among accessions obtained with RAPD markers was higher (79%) compared to ISSR. The genetic distance values were also larger than ISSR data ranging from 0.16 to 0.61 with 50% of values smaller than 0.35. The mean level of polymorphisms within the accessions analyzed was 65%. The Nei’s gene diversity (h) was 0.23. Overall, the genetic variation among and within QPM and normal varieties were high, but the genetic distance among them was small. QPM accessions clustered together, but the genetic closeness among QPM and normal maize varieties studied was not always related to their origin.
机译：近年来，以提高玉米籽粒蛋白质的营养价值为目标的优质蛋白质玉米（QPM）的使用已成为一些非洲育种计划的重点。了解QPM和正常玉米品种之间的遗传变异对于有效选择和开发新品种至关重要。本研究的主要目的是评估DR中选择的QPM和正常玉米品种之间以及内部的遗传变异水平和相关性。 -使用简单重复序列（ISSR）和“随机扩增多态性DNA”（RAPD）标记的刚果育种计划。对于ISSR分析，每个被分析种质的平均多态性水平为69％。 Nei的基因多样性（h）平均为0.26。这些种之间的遗传距离从0.098到0.460不等。超过80％的遗传距离低于0.35。与ISSR相比，使用RAPD标记获得的种质中多态性基因座的平均水平更高（79％）。遗传距离值也大于ISSR数据，范围为0.16至0.61，其中50％的值小于0.35。分析的种质内多态性的平均水平为65％。 Nei的基因多样性（h）为0.23。总体而言，QPM与正常品种之间和内部的遗传变异较高，但它们之间的遗传距离较小。 QPM的种质聚集在一起，但是QPM与研究的正常玉米品种之间的遗传亲缘关系并不总是与其起源有关。

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