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首页> 外文期刊>African Journal of Biotechnology >Cloning and characterization of cDNA encoding xyloglucan endotransglucosylase in Pennisetum glaucum L.
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Cloning and characterization of cDNA encoding xyloglucan endotransglucosylase in Pennisetum glaucum L.

机译：青草狼尾草木葡聚糖内切葡糖基化酶编码cDNA的克隆与鉴定

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摘要

Biomass production in plant is directly related to the amount of intercepted solar radiation by the canopy and available water to the plant. Growth and development of leaves, especially under drought condition, is therefore major determinant of crop productivity. Xyloglucan endotransglucosylase (XET) plays important role in growth and development of plants. XETs are a family of enzymes that mediate construction and restructuring of xyloglucan cross-links, thereby controlling the mechanical properties of cell wall. We cloned complete cDNA of an XET from pearl millet (Pennisetum glaucum?L.) and characterized it using?in silico?comparative genomics and activity assays. The cloned cDNA was 1266 bp in length, encoding a protein with 291 amino acids having signal peptide targeting it to the cell wall. The protein showed xyloglucan endotransglucosylase activity but no hydrolytic activity, therefore, named as?PgXET1?as per the convention. The comparative genomics revealed that the functional sites of the enzyme (XET) were highly conserved. Evolutionary studies using phylogenetic tree indicated its grouping with XETs from maize (with >95% bootstrap support), barley, rice, etc. This is the first report on cloning and characterization of an XET (PgXET1) from pearl millet, an important dual-purpose crop.
机译：植物中的生物量生产与冠层截获的太阳辐射量和植物可用的水量直接相关。因此，叶片的生长和发育，特别是在干旱条件下，是决定作物生产力的主要因素。木葡聚糖内转葡糖基化酶（XET）在植物的生长和发育中起重要作用。 XET是一类酶，可介导木葡聚糖交联的构建和重组，从而控制细胞壁的机械性能。我们从珍珠粟（Pennisetum glaucum？L。）中克隆了XET的完整cDNA，并在计算机模拟比较基因组学和活性分析中对其进行了表征。克隆的cDNA长度为1266 bp，编码具有291个氨基酸的蛋白质，该蛋白质具有将其靶向细胞壁的信号肽。该蛋白质显示木葡聚糖内转葡糖基化酶活性，但没有水解活性，因此按照惯例命名为“ PgXET1”。比较基因组学表明，该酶（XET）的功能位点是高度保守的。使用系统树的进化研究表明，它与来自玉米（> 95％的自举支持），大麦，大米等的XET分组。这是关于从小米中分离出XET（PgXET1）的第一份报道，这是重要的双重目的作物。

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