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首页> 外文期刊>African Journal of Biotechnology >Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design
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Factors affecting endoglucanase production by Trichoderma reesei RUT C-30 from solid state fermentation of oil palm empty fruit bunches using Plackett-Burman design

机译：使用Plackett-Burman设计影响里氏木霉RUT C-30固态发酵油棕空果串而产生内切葡聚糖酶的因素

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摘要

A study was conducted to screen parameters affecting the production of endoglucanase by?Trichoderma reesei?RUT C-30 on solid state fermentation of oil palm empty fruit bunch using the Plackett-Burman design. Factors involved in the screening process? were peptone concentration, urea concentration, ammonium sulfate concentration, calcium nitrate concentration, yeast extract concentration, tween 80 concentration, pH, incubation time, initial moisture content, inoculum size and substrate amount. Through analysis of variance (ANOVA), it was found that initial moisture content (p= 0.001), incubation time (p= 0.001), inoculum size (p= 0.032) and ammonium sulfate concentration (p= 0.023) have been recognized as significant factors affecting?endoglucanase activity in solid state fermentation (SSF) of oil palm empty fruit bunch (OPEFB) by?T. reesei?RUT C-30. The model established from the ANOVA analysis have a significant value of Pmodel>F=?0.0008 and?R2?of?0.9132. The pre-optimized media showed 2.6 fold increased of endoglucanase activity.
机译：进行了一项研究，以采用Plackett-Burman设计的方法筛选影响里氏木霉RUT C-30对油棕空果束固态发酵产生内切葡聚糖酶的参数。筛选过程涉及哪些因素？分别为蛋白concentration浓度，尿素浓度，硫酸铵浓度，硝酸钙浓度，酵母提取物浓度，吐温80浓度，pH，孵育时间，初始水分含量，接种量和底物量。通过方差分析（ANOVA），发现初始水分含量（p = 0.001），孵育时间（p = 0.001），接种量（p = 0.032）和硫酸铵浓度（p = 0.023）被认为是有效的。 T对油棕空果束（OPEFB）固态发酵（SSF）中链内葡聚糖酶活性的影响reesei？RUT C-30。通过ANOVA分析建立的模型具有显着的值，Pmodel＞ F ＝Δ0.0008和ΔR2Δ为0.9132。预先优化的培养基显示出内切葡聚糖酶活性增加了2.6倍。

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