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首页> 外文期刊>African Journal of Biotechnology >A two-dimensional electrophoresis protocol suitable for Medicago truncatula leaf proteome
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A two-dimensional electrophoresis protocol suitable for Medicago truncatula leaf proteome

机译：适用于t藜苜蓿叶片蛋白质组的二维电泳方案

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Medicago truncatula?leaves were used as the experimental materials. Total proteins of leaves were extracted by trichloracetic acid (TCA)-acetone method and proteins had a better separation using gel strips, forming an immobilized non-linear 3 to 10 pH gradient focusing 123,000 vhr combined with 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The gels were stained with Coomassie Brilliant Blue G-250 and digitalized gels were analyzed using the PDquest 8.0.1 software. The results indicated that 931 protein dots were detected in the gel. A technology suitable for the?M. truncatula?leaves protein extraction by TCA/acetone and a protocol for two-dimensional electrophoresis (2-DE) was established, which provides technical support for?M. truncatula?leaf proteome research.
机译：紫花苜蓿叶用作实验材料。通过三氯乙酸（TCA）-丙酮法提取叶片中的总蛋白质，并使用凝胶条更好地分离蛋白质，形成固定的非线性3至10 pH梯度，聚焦123,000 vhr，结合12％十二烷基硫酸钠聚丙烯酰胺凝胶电泳（ SDS-PAGE）。凝胶用考马斯亮蓝G-250染色，并使用PDquest 8.0.1软件分析数字化凝胶。结果表明在凝胶中检测到931个蛋白质点。一种适合于M的技术。截叶提取物通过TCA /丙酮提取蛋白质，并建立了二维电泳（2-DE）协议，为？M提供了技术支持。 truncatula？叶蛋白质组学研究。

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