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首页> 外文期刊>African Journal of Biotechnology >Proteome level changes in the root of Brassica alboglabra induced by alachlor herbicide
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Proteome level changes in the root of Brassica alboglabra induced by alachlor herbicide

机译：甲草胺除草剂诱导小白菜根部蛋白质组水平的变化

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摘要

Chinese kale (Brassica alboglabra) is a famous and extensively grown vegetable in Southeast Asia. Despite its nutritional values, pesticides are heavily applied to it. In this study, changes in protein expression due to alachlor treatment on?B. alboglabrawere investigated by using the 2-dimensional PAGE. Differential protein expressions were determined by using Image Master Software with volume (%) ≥2 fold as significant. Ten spots of interest have been identified by LC/MS/MS showing significant increases in differential protein expression between?B. alboglabra?roots treated with alachlor as compared to the untreated group which include HSC-cognate binding proteins, adenosylmethionine synthetase and beta-tubulin involved in defence mechanism in plants. Little is known about the function of other proteins identified which include knox-like proteins and hypothetical protein. Further investigations on plant proteomics would provide more information on the effects of different types of pesticides.
机译：芥蓝（Brassica alboglabra）是东南亚著名且广泛种植的蔬菜。尽管具有营养价值，但仍大量使用农药。在这项研究中，由于甲草胺处理？B而引起的蛋白质表达变化。通过使用二维PAGE研究了玻璃纤维。通过使用Image Master Software确定差异蛋白质表达，其体积（％）≥2倍。通过LC / MS / MS已经鉴定出十个感兴趣的斑点，它们表明ΔB之间的差异蛋白表达显着增加。与未处理组相比，用甲草胺处理过的草状根包括与植物防御机制有关的HSC-同源结合蛋白，腺苷甲硫氨酸合成酶和β-微管蛋白。对于鉴定出的其他蛋白质的功能知之甚少，这些蛋白质包括诺克斯样蛋白质和假定蛋白质。对植物蛋白质组学的进一步研究将提供有关不同类型农药作用的更多信息。

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