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首页> 外文期刊>African Journal of Biotechnology >Identification of a -galactosidase fruit pulp-specific promoter and its use in silencing constructs to reduce fruit softening in papaya
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Identification of a -galactosidase fruit pulp-specific promoter and its use in silencing constructs to reduce fruit softening in papaya

机译：-半乳糖苷酶果肉特异性启动子的鉴定及其在沉默构建物中的应用以减少木瓜中的果实软化

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摘要

β-Galactosidases have been proposed as key hydrolytic enzymes involved in cell wall degradation and rapid softening during postharvest processing and storage of papaya. To genetically improve the shelf life of papaya, we identified a softening-related?β-Galgene in fruit pulp at the 50% pericarp yellowing stage and isolated a novel?β-Galpromoter region. An online database search predicted that the core promoter motifs and?cis-acting elements in the isolated sequence were related to phytohormones, particularly to ethylene and stress responsiveness. GUS staining revealed different patterns of transient?GUS?expression in papaya organs driven by the putative promoter, with the highest level in the fruit pulp followed by the embryo and the root. Further, such expression was wound inducible in vascular tissues. Co-transformation of the two T-DNAs was performed, mediated by?Agrobacterium tumefaciensharboring a plant expression vector with the fruit pulp-specific promoter and an inverted repeat?β-Gal?cassette in one T-DNA and marker genes in the other T-DNA. A total of 24 regenerated plantlets were obtained, one of which was identified to be co-transformed using GUS staining, PCR assay and Southern blotting.
机译：已经提出β-半乳糖苷酶是参与番木瓜采后加工和储存期间细胞壁降解和快速软化的关键水解酶。为了从基因上改善木瓜的货架期，我们在果皮变黄50％的过程中在果肉中鉴定了与软化相关的β-Galgene，并分离了一个新的β-Galpromoter区。在线数据库搜索预测，分离序列中的核心启动子基序和顺式作用元件与植物激素有关，特别是与乙烯和胁迫响应性有关。 GUS染色揭示了在木瓜器官中由假定启动子驱动的瞬时GUS表达的不同模式，在果肉中含量最高，其次是胚芽和根。此外，这种表达在血管组织中可被伤口诱导。通过根癌农杆菌介导的植物表达载体与果肉特异性启动子和一个T-DNA中的反向重复β-Gal？盒以及另一个T中的标记基因介导了两个T-DNA的共转化。 -脱氧核糖核酸。总共获得了24个再生的小植株，其中之一经GUS染色，PCR测定和Southern印迹鉴定为共转化的。

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