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首页> 外文期刊>African Journal of Biotechnology >Isolation and characterization of anticoagulant compound from marine mollusc Donax faba (Gmelin, 1791) from Thazhanguda, Southeast Coast of India
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Isolation and characterization of anticoagulant compound from marine mollusc Donax faba (Gmelin, 1791) from Thazhanguda, Southeast Coast of India

机译：来自印度东南海岸塔章达（Thazhanguda）的海洋软体动物Donax faba（Gmelin，1791）的抗凝血化合物的分离和表征

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Glycosaminoglycans (GAGs) are linear polysaccharides found in the extracellular matrix and biological fluids of animals where they interact with hundreds of proteins and perform a variety of critical roles. There are five classes of animal GAGs: heparan sulfate (HS), chondroitin sulfate (CS), dermatan sulfate (DS), keratan sulfate (KS) and hyaluronan (HA). Many biological functions can be monitored directly by their impact on GAG quantity. In the present study, glycosaminoglycans were isolated from marine bivalve?Donax faba.?The amount of crude GAG was estimated as?12 gm/kg and?of tissue in?D. faba. After purification using gel chromatography, the yield was found to be?0.83 mg/kg.?The bivalve showed the anticoagulant activity of the crude and purified samples 58 and 114 USP units/mg correspondingly in?D. faba. The structural characterization of anticoagulant GAG was analyzed by Fourier transform infrared spectroscopy. Among the marine bivalve,?D. faba?purified showed more anticoagulant activity than that of?crude sample. The results of this study suggest that the GAG fromD. faba?could be an alternative source of heparin.
机译：糖胺聚糖（GAG）是在动物的细胞外基质和生物体液中发现的线性多糖，它们与数百种蛋白质相互作用并发挥多种关键作用。动物GAG有五类：硫酸乙酰肝素（HS），硫酸软骨素（CS），硫酸皮肤素（DS），硫酸角质素（KS）和透明质酸（HA）。许多生物功能可以通过其对GAG量的影响来直接监控。在本研究中，从海生双壳类动物“ Donax faba”中分离出糖胺聚糖。粗制GAG的量估计为12 gm / kg，组织中的组织为D。蚕豆用凝胶色谱法纯化后，发现收率为〜0.83mg / kg。双壳类动物相应地在ΔD中显示出粗品和纯化样品58和114USP单位/ mg的抗凝活性。蚕豆抗凝GAG的结构表征通过傅里叶变换红外光谱分析。在海洋双壳类动物中，？蚕豆纯化后的抗凝血活性比粗样品高。这项研究的结果表明GAG来自D。法巴？可能是肝素的替代来源。

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