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首页> 外文期刊>African Journal of Biotechnology >Estimation of genetic diversity of the Kenyan yam (Dioscorea spp.) using microsatellite markers
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Estimation of genetic diversity of the Kenyan yam (Dioscorea spp.) using microsatellite markers

机译：利用微卫星标记估算肯尼亚山药（Dioscorea spp。）的遗传多样性

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Yam landraces in Kenya have not been fully characterized both at morphological and molecular level. Application of molecular markers can overcome this bottleneck. 187 accessions comprising of 166 yam landraces and 21 Yam DNA samples from IITA, Nigeria were extracted from leaf samples grown at Muguga and genotyped at BeCA. DNA was extracted using CTAB method. Twelve (12) primer pairs were used for genotyping and PCR products detected on ABI-3730 capillary system. Data was analyzed for genetic diversity, ordination and analysis of molecular variance with GenAIEx software. A total of 131 alleles were amplified with a minimum of two alleles and a maximum of 13 alleles per primer with a minimum allele size of 64 bp and a maximum of 368 bp. Accessions from Eastern province had the highest number of unique alleles. Shannon’s information index (I) was 0.1444 for West African samples and 0.2366 for Central province. Accession dispersion revealed four clusters with no distinct geographical pattern. Dense clustering of accessions was an indication of genetic relatedness.?Analysis of molecular variance revealed that most variation of 88% (P<0.010) was found within populations or provinces. The?simple sequence repeats (SSR) markers were polymorphic and were able to discriminate local yam landraces.
机译：肯尼亚的山药地方品种尚未在形态和分子水平上得到充分表征。分子标记的应用可以克服这一瓶颈。从尼日利亚穆塔加（Muguga）生长并在BeCA进行基因分型的叶样品中提取了来自尼日利亚IITA的187种材料，其中包括166种山梨种和21种Yam DNA样品。用CTAB法提取DNA。使用十二（12）对引物进行基因分型，并在ABI-3730毛细管系统上检测到PCR产物。使用GenAIEx软件分析数据的遗传多样性，排序和分子变异分析。总共131个等位基因被扩增，每个引物最少两个等位基因，最多13个等位基因，最小等位基因大小为64 bp，最大为368 bp。来自东部省份的种质的独特等位基因数量最多。西非样品的香农信息指数（I）为0.1444，中部省为0.2366。入藏分散显示四个簇，没有明显的地理格局。种质的密集聚类表明了遗传的相关性。对分子变异的分析表明，大多数变异发生在88％（P <0.010）的人群或省份内。简单序列重复（SSR）标记是多态的，能够区分本地山药地方品种。

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