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首页> 外文期刊>African Journal of Biotechnology >Arsenic, boron and salt resistant Bacillus safensis MS11 isolated from Mongolia desert soil
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Arsenic, boron and salt resistant Bacillus safensis MS11 isolated from Mongolia desert soil

机译：从蒙古沙漠土壤中分离出来的抗砷，硼和盐分抗性芽孢杆菌MS11

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Arsenic?(As)?is a toxic metalloid, having both properties of a metal and a non-metal. Boron?(B)?is a non-metal, essential micronutrient for plants growth and development but its?excess can?be?toxic to plants with various levels. In this study arsenic, boron and salt resistant bacteria were isolated from desert soil, Mongolia. The bacterial screening was carried out by serial dilution method. One hundred colonies were screened initially?using?of?(2 mM)?arsenic?and?(2 mM)?boron?containing LB?agar medium.?From this?population,?10 bacterial isolates were selected based on arsenic and boron resistance, boron accumulation, salt tolerance and arsenic oxidizing capability. One of the potent?strain,?MS 11?from Mongolia desert soil,?was?tentativelyidentified?as?Bacillus?sp. The phylogenetic and comparative analysis of 16S rRNA gene sequence with closely related validly published species available in the database showed that the isolate MS11 was closely related to?Bacillus safensis?FO-036b(T) with the highest sequence similarity (99.439%). The 16S rRNA gene sequence of the strain MS11 was submitted in the NCBI database with the accession number JF836885.?B. safensis?MS11 exhibited high level of resistance to?arsenite (40 mM),arsenate (400 mM), boron (200 mM) and 15% salt tolerance in LB agar medium.?B. safensis?MS11?was?also?associated with?resistance to?multiple?heavy metals?such as Cd,?Cr, Cu, Ni, Pb and Zn.?Hence, this bacterium could be useful in the remediation of salt affected soils and biogeochemical cycles of arsenic?pollution.
机译：砷（As）2是一种有毒的准金属，具有金属和非金属的特性。硼（B）是植物生长和发育的一种非金属必需微量营养素，但其过量可能对各种含量的植物有毒。在这项研究中，从蒙古沙漠土壤中分离出了耐砷，硼和盐分的细菌。通过系列稀释法进行细菌筛选。首先使用（2 mM）砷和（2 mM）硼（含LB）琼脂培养基筛选了一百个菌落。从该种群中，基于砷和硼选择了10种细菌分离株。耐性，硼积累，耐盐性和砷氧化能力。来自蒙古沙漠土壤的一种强力菌株MS 11初步鉴定为芽孢杆菌。对16S rRNA基因序列与数据库中有效关联的密切相关物种进行的系统发育和比较分析表明，分离物MS11与？枯草芽孢杆菌？FO-036b（T）密切相关，序列相似性最高（99.439％）。 MS11菌株的16S rRNA基因序列已在NCBI数据库中提交，登录号为JF836885.?B。 safensis®MS11在LB琼脂培养基中表现出对亚砷酸盐（40 mM），砷酸盐（400 mM），硼（200 mM）的高水平抗性和15％的耐盐性。安全性MS11还与对多种重金属（如Cd，Cr，Cu，Ni，Pb和Zn）的抗性相关。因此，该细菌可用于修复盐碱土和土壤。砷污染的生物地球化学循环。

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