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首页> 外文期刊>African Journal of Biotechnology >Induction of somatic embryogenesis and plant regeneration in the reed grass (Phragmites communis Trin.)
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Induction of somatic embryogenesis and plant regeneration in the reed grass (Phragmites communis Trin.)

机译：芦苇草（Phragmites communis Trin。）诱导体细胞胚发生和植物再生。

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An?in vitro?culture system for the large-scale propagation of?Phragmites communisTrin.?(reed)?was established by optimizing culture conditions for callus induction and differentiation together with plant?propagation using regenerated plantlets. Callus was induced from stem segments with callus induction medium containing auxin: 4-fluorophenoxyacetic acid (4-FA) or 2,4-dichlorophenoxy?acetic acid (2,4-D). A high frequency of callus induction was observed at relatively low concentrations (0.5 and 1 mg L-1) of both 2,4-D or 4-FA. However, high concentrations (3, 4 and 5 mg L-1) of either auxin suppressed callus induction. When applied for the first time, 1.0 mg L-1?4-FA markedly improved the frequency of callus induction (up to 93%). The callus was then transferred to MS medium supplemented with 1.0 mg L-1?2,4-D to promote the formation of embryogenic calli. The calli were grown in MS supplemented with different concentrations of 2,4-D and naphthaleneacetic acid (NAA) alone or in combination with benzyladenine (BA). After seven weeks of culture, the regeneration efficiency was determined for the calli maintained for the 45 differentiation media formulations. The highest regeneration capacity was obtained from the medium containing 0.05 mg L-1?NAA and 2 mg L-1?BA, and the combination of 0.2 mg L-1?NAA and 2 mg L-1?BA. Propagation of the regenerated plantlets was also examined in medium containing different sucrose concentrations; this experiment found that 60 g L-1?sucrose showed the best growth rate. These improved regeneration and propagation systems could be used for bioreactor-based mass propagation or an?in vitro?culture system, and would be useful for transformation in?Phragmites communisTrin.
机译：通过优化愈伤组织的诱导和分化培养条件，并利用再生小植株进行繁殖，建立了芦苇大规模繁殖的体外培养系统。用含有生长素的愈伤组织诱导培养基从茎节诱导愈伤组织：4-氟苯氧基乙酸（4-FA）或2,4-二氯苯氧基乙酸（2,4-D）。在2,4-D或4-FA的相对较低浓度（0.5和1 mg L-1）下观察到高频率的愈伤组织诱导。但是，任何一种生长素的高浓度（3、4和5 mg L-1）都会抑制愈伤组织的诱导。首次使用时，1.0 mg L-1？4-FA可以显着提高愈伤组织的诱导频率（高达93％）。然后将愈伤组织转移到补充有1.0mg L-1β2，4-D的MS培养基中，以促进胚性愈伤组织的形成。愈伤组织在补充有不同浓度的2,4-D和萘乙酸（NAA）的MS中或与苄基腺嘌呤（BA）组合的MS中生长。培养七周后，确定对于45种分化培养基制剂保持的愈伤组织的再生效率。从含有0.05mgL-1αNAA和2mgL-1βBA以及0.2mgL-1αNAA和2mgL-1βBA的组合的培养基中获得最高的再生能力。还应在含有不同蔗糖浓度的培养基中检查再生苗的繁殖。该实验发现60 g L-1？蔗糖显示出最佳的生长速率。这些改进的再生和繁殖系统可用于基于生物反应器的大规模繁殖或“体外”培养系统，并且可用于在芦苇中转化。

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