• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Efficient somatic embryo production of Limau madu (Citrus suhuiensis Hort. ex Tanaka) in liquid culture
【24h】

Efficient somatic embryo production of Limau madu (Citrus suhuiensis Hort. ex Tanaka) in liquid culture

机译：液态培养中Limau madu（Citrus suhuiensis Hort。ex Tanaka）的体细胞胚高效生产

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Effects of N6-benzylaminopurine (BAP) concentration, initial cell density and carbon sources and concentrations for producing cell suspension and somatic embryos of Limau madu (Citrus suhuiensis?Hort. ex Tanaka) were investigated using cell suspension culture. Cells were first inoculated into Murashige and Skoog (MS) medium (1962) supplemented with 4.4 to 13.3 μM BAP. Growth rate of cells was at its maximum (6.69 mg day-1) in media supplemented with a lower concentration of BAP (6.7 μM). Embryogenic cell at 2 mg ml-1?was found to be the most effective inoculum size for the highest growth rate (3.35 mg day-1) of cell proliferation within a period of 15 to 30 days after inoculation (DAI). This inoculum size resulted in 31.75% faster embryo growth than those with inoculum densities of 4 to 6 mg ml-1. Sucrose (88, 117 and 146 mM), glycerol (16, 22 and 27 mM) and combinations of sorbitol and galactose (146:0, 110:36, 73:73, 36:110 and 0:146 mM) were tested for their effects on embryogenic cell proliferation and somatic embryo induction. Results indicates that sucrose at 146 mM induced cell proliferation (7.65 mg day-1) and produced a higher quantity of cells than glycerol at 27 mM (2.33 mg day-1) and a combination of sorbitol and galactose at 73:73 mM (4.64 mg day-1), but failed to induce somatic embryos. Glycerol in different concentrations was ineffective in cell proliferation and somatic embryo induction. At optimal BAP concentration (6.7 μM), a small amount of embryogenic cells (100 mg in 50 ml) can be multiplied profusely in sucrose-containing medium. A large number of somatic embryos (951) were induced in a medium containing 110 mM sorbitol and 36 mM galactose as the most effective carbon source for inducing somatic embryos without BAP.
机译：研究了N6-苄基氨基嘌呤（BAP）浓度，初始细胞密度和碳源的浓度对生产Limau madu（Citrus suhuiensis？Hort。ex Tanaka）细胞悬浮液和体细胞胚的浓度的影响。首先将细胞接种到补充有4.4至13.3μMBAP的Murashige和Skoog（MS）培养基中（1962）。在补充了较低浓度的BAP（6.7μM）的培养基中，细胞的生长速率达到最大值（6.69 mg day-1）。发现在接种（DAI）后的15到30天内，以2 mg ml-1？的胚性细胞是最有效的接种物，其细胞增殖最高（3.35 mg day-1）。与接种密度为4至6 mg ml-1的那些相比，这种接种物的大小可导致胚胎生长快31.75％。测试了蔗糖（88、117和146 mM），甘油（16、22和27 mM）以及山梨糖醇和半乳糖的组合（146：0、110：36、73：73、36：110和0：146 mM）它们对胚胎发生细胞增殖和体细胞胚诱导的影响。结果表明，蔗糖浓度为146 mM时诱导细胞增殖（7.65 mg第1天），产生的细胞数量高于甘油浓度为27 mM时（2.33 mg day-1）以及山梨糖醇和半乳糖的组合浓度为73:73 mM（4.64）。毫克第1天），但未能诱导体细胞胚。不同浓度的甘油对细胞增殖和体细胞胚诱导无效。在最佳BAP浓度（6.7μM）下，可以在含蔗糖的培养基中大量繁殖少量胚发生细胞（100 mg在50 ml中）。在包含110 mM山梨糖醇和36 mM半乳糖的培养基中诱导了大量的体细胞胚（951），这是诱导没有BAP的体细胞胚最有效的碳源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号