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首页> 外文期刊>African Journal of Biotechnology >Effect of inhibiting the expression of insulin-like peptide gene BBX-B8 on development and reproduction of silkworm, Bombyx mori
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Effect of inhibiting the expression of insulin-like peptide gene BBX-B8 on development and reproduction of silkworm, Bombyx mori

机译：抑制胰岛素样肽基因BBX-B8的表达对家蚕发育和繁殖的影响

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摘要

Bombyxin (BBX) of the silkworm,?Bombyx mori, is an insulin-like peptide (ILP). To further study the function of the?BBX-B8 gene, the effects of inhibiting the expression of?BBX-B8 gene on organ development, reproduction and trehalose metabolization of the silkworm were investigated. 48 h after the injection of?BBX-B8 dsRNA into the 3-day larvae of fifth-instar (10 μg per larvae; 10 μl; the average weight: 3 g/larvae), the mRNA level of?BBX-B8 in the brain of silkworm was declined strikingly by 37.33%; 72 h after injection, the mRNA level of BBX-B8 was decreased by 30%. After the injection of?BBX-B8 dsRNA into the 3-day larvae of fifth-instar (1 or 5 μg per larvae; 10 μl), development of pupal wing imaginal discs was suppressed, more deformed adult wings were found. The number of mature eggs in the oviduct respectively increased by 7.86 and 12.62% when pupa were injected with?BBX-B8 dsRNA (10 or 15 μg per larvae; 10 μl) on the first day of pupation. 72 h after the injection of?BBX-B8 dsRNA (10 μg per larvae; 10 μl) into the 3-day larvae of fifth-instar, the trehalose level of hemolymph was elevated by 12.68% in silkworm. These results suggest that BBX-B8 plays an important role in organ development, reproduction and the metabolization of trehalose of silkworm.
机译：家蚕的家蚕毒素（BBX）是一种胰岛素样肽（ILP）。为了进一步研究βBBX-B8基因的功能，研究了抑制βBBX-B8基因表达对家蚕器官发育，繁殖和海藻糖代谢的影响。将？BBX-B8 dsRNA注射至五龄幼虫的3天幼虫（每个幼虫10μg; 10μl;平均重量：3 g /幼虫）后48小时，小鼠体内BBX-B8的mRNA水平蚕脑显着下降37.33％;注射72小时后，BBX-B8的mRNA水平降低了30％。将BBBB-B8 dsRNA注射至五龄幼虫的3天幼虫（每个幼虫1或5μg; 10μl）后，p翼假想盘的发育受到抑制，发现成年后的翅膀变形更多。在化up的第一天，用？BBX-B8 dsRNA（每个幼虫10或15μg; 10μl）注射，输卵管中成熟卵的数量分别增加了7.86和12.62％。在五龄幼虫的3天幼虫中注射？BBX-B8 dsRNA（每个幼虫10μg; 10μl）后72小时，家蚕中的血淋巴海藻糖水平提高了12.68％。这些结果表明，BBX-B8在家蚕的器官发育，繁殖和海藻糖代谢中起重要作用。

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