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首页> 外文期刊>African Journal of Biotechnology >Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica
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Induction of apoptosis in human breast cancer cell line MCF-7 by phytochemicals from Gmelina asiatica

机译：积雪草中的植物化学物质诱导人乳腺癌细胞MCF-7凋亡

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摘要

Currently, breast cancer is the leading cause of cancer-related death in women. Therefore, there is an urgent need to develop alternative therapeutic measures against this deadly disease.?Many components from dietary or medicinal plants have been identified that possess substantial chemopreventive properties. India has unique plant varieties yet to be studied for anticancer components. Therefore,cytotoxicity activity and the mechanism of cell death exhibited by the extracts prepared from?Gmelina asiatica, in human breast cancer MCF-7cells was investigated. The MCF-7 cells were seeded in 96-well culture plates in the presence and absence of different concentrations of extracts of?G. asiatica?to determine their anticancer effects using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)?assay. Mechanism of cell death was evaluated by chromatin condensation using Hoest staining and morphological changes with the use of a contrast microscope.?Plant extracts of?G. asiatica?were observed to induce apoptosis of MCF-7 cells as evidenced by MTT-cell proliferation assay, cell-morphological changes and chromatin condensation.?The cytotoxicity of the chloroform extract was greater than other extracts of?G. asiatica. The results of the present investigation is the first report on the potential anticancer activity of?G. asiatica?extracts and its possible mechanism of action on cancer cell proliferation in breast cancer MCF-7 cell lines.?Key?words:?Breast cancer, MCF-7 cells, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, a tetrazole assay, apoptosis,?Gmelina asiatica.
机译：当前，乳腺癌是女性与癌症相关的死亡的主要原因。因此，迫切需要开发针对这种致命疾病的替代性治疗措施。已经发现来自饮食或药用植物的许多成分具有实质性的化学预防特性。印度有独特的植物品种，尚需研究其抗癌成分。因此，研究了由积雪草提取物制备的提取物对人乳腺癌MCF-7细胞的细胞毒性活性和细胞死亡机理。在存在和不存在不同浓度的βG提取物的情况下，将MCF-7细胞接种在96孔培养板中。积雪草使用3-（4,5-二甲基噻唑-2-基）-2,5-二苯基溴化四氮唑（MTT）测定法来确定其抗癌作用。通过使用Hoest染色的染色质浓缩和使用对比显微镜的形态变化来评估细胞死亡的机制。通过MTT-细胞增殖测定，细胞形态变化和染色质浓缩观察到了积雪草可诱导MCF-7细胞凋亡。氯仿提取物的​​细胞毒性大于其他提取物。积雪草。本研究的结果是关于？G潜在抗癌活性的首次报道。积雪草提取物及其对乳腺癌MCF-7细胞系癌细胞增殖的可能作用机制。关键词：乳腺癌，MCF-7细胞，3-（4,5-二甲基噻唑-2-基）- 2,5-二苯基溴化四氮唑，四唑测定，细胞凋亡，积雪草。

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