• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Biosynthesis of polyhydroxybutyrate in Enterobacter sp. SEL2 and Enterobacteriaceae bacterium sp. PFW1 using sugar cane molasses as media
【24h】

Biosynthesis of polyhydroxybutyrate in Enterobacter sp. SEL2 and Enterobacteriaceae bacterium sp. PFW1 using sugar cane molasses as media

机译：肠杆菌属中多羟基丁酸酯的生物合成。 SEL2和肠杆菌科细菌sp。 PFW1以甘蔗糖蜜为培养基

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present research was conducted to check the cane molasses as a media for the production of polyhydroxybutyrate?(PHB). Different dilutions of cane molasses were used in combination with water and mineral medium and in different ratios of carbon and nitrogen sources. Out of?the?54?bacteria isolated from three types of organic waste contaminated environments, two were selected for their highest intensity of fluorescence under UV by Nile blue A viable colony staining method and they produced maximum amount of PHB from glucose (66.61±0.05% and 76.92±0.04%) in a shake flask culture at pH 7.0, 37°C and 150 rpm. The bacterial strains were identified as Gram negative rods and the 16S ribosomal ribonucleic acid (RNA) sequence similarity search showed 99 and 98% homology to?Enterobacter?sp. andEnterobacteriaceae bacterium?respectively. In bacterial strains?Enterobacter?sp. SEL2 (JF901810) and?Enterobacteriaceae bacterium?PFW1 (JF901811), the polyhydroxybutyrate (PHB) biosynthesis from molasses was observed as 57.61±0.57% and 58.07±0.25% respectively at pH 7.0, 37°C and 150 rpm. The optimal time was 24 to 48 h for strain SEL2 and 48 to 72 h for strain PFW1. The best growth and polyhydroxyalkanoates (PHA) production was observed in media with 2% molasses and 0.2% ammonium sulphate in mineral medium (Media 11). The functional groups of the extracted PHA granules were identified as C=O group by Fourier transform infrared (FTIR) spectroscopy analysis and proton nuclear magnetic resonance (NMR) analysis confirmed it as a homopolymer of hydroxybutyrate.
机译：进行本研究以检查甘蔗糖蜜作为生产聚羟基丁酸酯（PHB）的介质。甘蔗糖蜜的不同稀释度与水和矿物质培养基结合使用，并以不同比例的碳和氮源使用。从三种类型的受有机废物污染的环境中分离出的“ 54”细菌中，通过尼罗蓝A可行菌落染色法选择了两种在紫外线下具有最高荧光强度的细菌，它们从葡萄糖中产生最大的PHB（66.61±0.05）摇瓶培养液在pH 7.0、37°C​​和150 rpm的条件下（1％和76.92±0.04％）。细菌菌株被鉴定为革兰氏阴性棒，并且16S核糖体核糖核酸（RNA）序列相似性搜索显示与“肠杆菌” sp有99％和98％的同源性。和肠杆菌科细菌。在细菌菌株中，肠杆菌属。 SEL2（JF901810）和肠杆菌科细菌PFW1（JF901811）观察到，在pH 7.0、37°C​​和150 rpm下，由糖蜜生物合成的聚羟基丁酸酯（PHB）分别为57.61±0.57％和58.07±0.25％。菌株SEL2的最佳时间为24至48小时，菌株PFW1的最佳时间为48至72小时。在矿物培养基中，在含2％糖蜜和0.2％硫酸铵的培养基中，观察到最佳的生长和多羟基链烷酸酯（PHA）产生（培养基11）。通过傅里叶变换红外（FTIR）光谱分析将提取的PHA颗粒的官能团鉴定为C ＝ O基团，并且质子核磁共振（NMR）分析证实其为羟基丁酸酯的均聚物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号