• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >A PCR ITS-RFLP method for identifying fungal contamination of date palm (Phoenix dactylifera L.) tissue cultures
【24h】

A PCR ITS-RFLP method for identifying fungal contamination of date palm (Phoenix dactylifera L.) tissue cultures

机译：PCR ITS-RFLP方法鉴定椰枣（Phoenix dactylifera L.）组织培养的真菌污染

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Morphological and molecular procedures have been used in the study to characterize the most abundant fungal contaminants of date palm tissue cultures.?Aspergillusspecies were found to be the most frequently isolated species, followed by the species of?Alternaria?and?Penicillium. Internal Transcribed Spacer (ITS) products of the predominant fungi were analyzed by PCR amplification with ITS1 and ITS4 primers. The results of BLAST search of the ITS sequences revealed the identity of the fungal species. Three species were found to belong to the genus?Aspergillus?as?Aspergillus clavatus,?Aspergillus niger?and?Aspergillus terreus, while, two species were found for the genus?Alternaria?with?Alternaria alternata?and?Alternaria citri, and two species for the genus of?Penicillium?with?Penicillium expansum?and?Penicillium glabrum. The restriction fragment length polymorphism (RFLP) of the ITS amplicons was used to discriminate between fungal species and provides an alternative method to sequencing ITS products; both restriction endonucleases?EcoRI and?SmaI were used to digest ITS products, and three types of fragment patterns were detected:?A?type (one undigested fragment),?B?type (two fragments type) and?C?type (three fragments type). This is the first study which used ITS sequence and ITS-RFLP techniques as a rapid and reliable procedure for identification of date palm fungal contamination in the laboratories of tissue cultures in Iraq.
机译：该研究已使用形态学和分子学方法表征了枣椰子组织培养物中最丰富的真菌污染物。曲霉菌属是最常见的分离物种，其次是Al木属和青霉菌。通过使用ITS1和ITS4引物进行PCR扩增，分析了主要真菌的内部转录间隔物（ITS）产物。 BITS搜索ITS序列的结果揭示了真菌物种的身份。发现三个物种属于曲霉属，曲霉菌，黑曲霉和黑曲霉属，而找到了两个物种，分别是Alternaria，Alternaria alternata和Citry citri。青霉菌，扩张青霉菌和延寿青霉菌属。 ITS扩增子的限制性片段长度多态性（RFLP）用来区分真菌种类，为ITS产品测序提供了另一种方法。限制性内切酶EcoRI和SmaI都用于消化ITS产物，检测到三种类型的片段模式：A型（一个未消化的片段），B型（两个片段型）和C型（三种）。片段类型）。这是首次使用ITS序列和ITS-RFLP技术作为伊拉克组织培养实验室中快速，可靠的程序来识别枣椰子真菌污染的研究。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号