The aim of this study is to investigate and compare the histopathological effects of hair dye additives, 2-amino-5-nitrophenol (2A5NP) and 2-nitro-p-phenylendiamin (2NPPD) on cornea of neonates from pregnant rats that have been administered these additives subcutaneously. The study included 90 neonates of 26 nulligravida wistar-albino rats among which ten were given 100 mg/kg/day 2A5NP (Group I), ten rats received 150 mg/kg/day 2NPPD (Group II) and control rats received saline (Group III) injections subcutaneously between 7th and 15th gestational days. No sign of toxicity was observed during the treatment and there was no gross abnormality in both the study and control groups. Histopathological changes of cornea were seen in 22 of 30 newborn rats in Group I (73.4%), in 23 of 30 rats in Group II (76.7%) and only 5 of 30 rats in the control saline injected Group III (16.7%). Histopathological effect of the two additives were statistically significant when compared to the control group (Chi-square:27.63, p = 0.0001), but there was no difference between the effects of 2A5NP and 2NPPD additives on cornea (Chi-square:0.089, p = 0.766). The present experimental study on rats confirmed the histopathological effect of 2A5NP and 2NPPD on cornea beyond doubt. In the light of which, we can speculate that maternal exposure of hair dyes during pregnancy has some teratogenic effects on newborn rat cornea.
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机译：这项研究的目的是调查和比较染发添加剂，2-氨基-5-硝基苯酚（2A5NP）和2-硝基-对-苯基联苯胺（2NPPD）对已给药的妊娠新生鼠的角膜的组织病理学影响。这些添加剂皮下注射。该研究包括90只新生动物，共26只零重力Wistar-albino大鼠，其中10只接受100 mg / kg / day 2A5NP（I组），十只大鼠接受150 mg / kg / day 2NPPD（II组），而对照大鼠接受生理盐水（Group I组） III）在妊娠第7至15天之间皮下注射。在治疗期间未观察到毒性迹象，研究组和对照组均未见明显异常。 I组的30只新生大鼠中的22只（73.4％），II组的30只大鼠中的23只（76.7％）和对照组注射盐水的III组的30只大鼠中的5只（16.7％）可见到角膜的组织病理学变化。与对照组相比，两种添加剂的组织病理学效果具有统计学意义（卡方：27.63，p = 0.0001），但2A5NP和2NPPD添加剂对角膜的作用无差异（卡方：0.089，p） = 0.766）。目前对大鼠的实验研究无疑证实了2A5NP和2NPPD对角膜的组织病理学作用。据此，我们可以推测孕妇在怀孕期间染发剂对新生大鼠角膜有致畸作用。
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