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首页> 外文期刊>African Journal of Biotechnology >Characterization of pathogen responsible for infection in bhindi plant by using phytoplasma specific universal primers
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Characterization of pathogen responsible for infection in bhindi plant by using phytoplasma specific universal primers

机译：使用植物原质特异性通用引物鉴定负责Bhindi植物感染的病原体

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Infection in bhindi plant (Abelmoschus esculentus, Family-Malvaceae) is very common in Gorakhpur district of Eastern U.P. region of India. To detect the pathogen, genomic DNA was isolated from the infected bhindi plant sample and amplified through polymerase chain reaction (PCR) by using P1 forward primer and Tint reverse primer, which were phytoplasma specific universal primers. Obtained PCR amplicons were cloned and sequenced. BLAST analysis discovered their 91% identity with the members of ‘Uncultured bacterium’. Phylogenetic tree also make their relationship with uncultured bacterium. Obtained nucleotide sequence was submitted to NCBI through accession number KF663567. To the best of our knowledge, this is the first report of “uncultured bacterium” (473 bp), from Gorakhpur district of Eastern U.P. region of India, associated with infection in bhindi plant.
机译：Bhindi植物（Abelmoschus esculentus，锦葵科）的感染在美国东部的Gorakhpur区非常普遍。印度地区。为了检测病原体，从感染的bhindi植物样品中分离出基因组DNA，并使用P1正向引物和Tint反向引物（是植物质体特异性通用引物）通过聚合酶链反应（PCR）进行扩增。克隆获得的PCR扩增子并测序。 BLAST分析发现它们与“未培养细菌”的成员具有91％的同一性。系统发育树也使它们与未培养的细菌有关。获得的核苷酸序列通过登录号KF663567提交给NCBI。据我们所知，这是来自美国东部戈拉克布尔地区的“未培养细菌”（473 bp）的首次报道。印度地区，与bhindi植物感染有关。

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