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首页> 外文期刊>African Journal of Biotechnology >Abnormal mitosis in root meristem cells of Allium cepa L. induced by a fabric dye reactive turquoise blue (Procion MX)
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Abnormal mitosis in root meristem cells of Allium cepa L. induced by a fabric dye reactive turquoise blue (Procion MX)

机译：织物染料活性翠蓝（Procion MX）引起的洋葱Cepa L.根分生组织异常有丝分裂

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Reactive Turquoise Blue (Procion MX) is a fabric dye used in small scale cotton fabric industries in various parts of India. The impact of this chemical on human health in the surrounding areas that discharge effluents is of serious concern. This needs to be assessed for short and long term effect on human genome. This investigation was aimed to find mitotic abnormalities as cytological evidence induced by the dye in root tip cells of onion (Allium cepa L.) grown in different concentrations: 0.01, 0.05, 0.1, 0.5 and 1.0% (weight per volume) prepared in distilled water in separate treatment schedules for 24 and 48 h. Mitotic aberrations (MA) were scored after staining with 2% acetocarmine by conventional squashing method. Root growth at various concentrations and duration of exposure of the dye were analyzed as macroscopic parameter for testing the cytotoxicity. Percentages of mitotic cells were analyzed as microscopic parameter to find the trend of mitotic indices and depression. Total abnormality of cells in percentage indicated the genotoxic assault of the dye. At higher concentrations, the root tip cells died in 24 h. Highest number of dividing cells with largest mitotic index value was observed at 0.01% exposed for 48 h. The abnormalities of common occurrences observed were unequal cytokinesis/ karyokinesis, formation of micronuclei, bi-nucleated cells and little condensed chromosomal arms in abnormal metaphase, anaphase and telophase. The abnormal mitotic cells were assumed to be due to genotoxic assault of the dye on chromosomal condensation mechanism resulting in very unusual long arms in rapidly dividing meristematic cells.
机译：活性绿松石蓝（Procion MX）是一种用于印度各地的小规模棉织物行业的织物染料。这种化学物质对周围环境中排放废水的人类健康的影响令人严重关注。需要评估这对人类基因组的短期和长期影响。这项研究的目的是寻找有丝分裂异常，作为染料在不同浓度（蒸馏后制备的浓度为0.01、0.05、0.1、0.5和1.0％（每体积重量）生长的洋葱）的根尖细胞中由染料诱导的细胞学证据。分别在24小时和48小时的处理时间内浇水。通过常规挤压法在用2％乙酰胭脂红染色后对有丝分裂畸变（MA）进行评分。作为测试细胞毒性的宏观参数，分析了染料在各种浓度和暴露持续时间下的根生长。分析有丝分裂细胞的百分比作为微观参数，以发现有丝分裂指数和抑郁的趋势。以百分比表示的细胞总异常表明该染料具有遗传毒性。在较高浓度下，根尖细胞在24小时内死亡。在暴露0.01％的情况下48小时观察到分裂细胞数量最多的分裂指数最大。在异常的中期，后期和末期，观察到的常见异常是胞质分裂/核运动不均等，微核，双核细胞的形成和染色体臂的浓缩。异常的有丝分裂细胞被认为是由于染料对染色体凝结机理的遗传毒性攻击导致在快速分裂的分生组织细胞中出现非常不寻常的长臂。

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