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首页> 外文期刊>African Journal of Biotechnology >Occurrence of Pospiviroid in potato, tomato and some ornamental plants in Turkey
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Occurrence of Pospiviroid in potato, tomato and some ornamental plants in Turkey

机译：土耳其马铃薯，番茄和一些观赏植物中含有类胡萝卜素

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In order to?investigate the?Pospiviroid?in potato, tomato and some ornamental plants in Turkey, a survey study was carried out from 2006 to 2009. During this survey study, a total of 1766 samples (1047 potato samples, 258 tomato leaves and 461 ornamental plants) were tested by reverse transcription-polymerase chain reaction (RT-PCR) and, the samples found to be positive in RT-PCR were also checked in return polyacrylamide gel electrophoresis (R-PAGE). Of these samples, potato tubers were collected from markets, leaf samples from?potato fields (Erzurum), tomato samples from field (Tokat, Amasya, Bal?kesir and Bursa) and greenhouses (Antalya and Mu?la), leaves and vines of ornamental plants from greenhouses (Yalova) and florists (Erzurum) and recreation areas (Ankara, ?zmir and Trabzon). In the result of RT-PCR test, PSTVd was detected in 6 out of 891 potato tubers and CSVd in 2 out of 154 chrysanthemums. On the other side, tomato, citrus and most ornamental plants belonging to different families were found to be free frompospiviroid. This is the first report of CSVd in Turkey.
机译：为了调查土耳其马铃薯，番茄和某些观赏植物中的“类胡萝卜素”，从2006年至2009年进行了一项调查研究。在此调查研究中，总共进行了1766份样品（1047份土豆样品，258片番茄叶和通过逆转录-聚合酶链反应（RT-PCR）测试了461株观赏植物），并在返回聚丙烯酰胺凝胶电泳（R-PAGE）中检查了RT-PCR中呈阳性的样品。在这些样品中，马铃薯块茎是从市场上采集的，马铃薯样品是从马铃薯田（Erzurum）采集的，番茄样品是从田间（Tokat，Amasya，Bal？kesir和Bursa）和温室（安塔利亚和穆拉）采集的，温室（雅洛瓦）和花店（埃尔祖鲁姆）和休闲区（安卡拉，兹米尔和特拉布宗）的观赏植物。在RT-PCR测试的结果中，在891个马铃薯块茎中有6个检出了PSTVd，在154个菊花中有2个检出了CSVd。另一方面，发现属于不同科的番茄，柑桔和大多数观赏植物中没有正痘病毒。这是土耳其CSVd的第一份报告。

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