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Lipase-producing fungi for potential wastewater treatment and bioenergy production

机译:产生脂肪酶的真菌,用于潜在的废水处理和生物能源生产

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The use of fungal biomass as a lipase biocatalyst represents an attractive approach for the treatments of oil wastewater as well as for the production of biodiesel from oil and residual grease, due to its greater stability, possibility of reuse, and lower cost. In this work, 20 filamentous fungi were isolated from the grease trap scum of a restaurant at the Federal University of Espírito Santo, Brazil. The fungi included those belonging to the genera Aspergillus, Beauveria, Botrytis, Cladosporium, Colletotrichum, Fusarium, Geotrichum, Penicillium, Rhizomucor, and Verticillium. Fungal lipase activity and biomass production were quantified. Lipase activity ranged from 0.13 U mg-1 protein of Rhizomucor sp. ECGF18 to 18.06 U mg-1 protein of Penicillium sp. ECGF02, and the biomass production ranged from 7.61 mg mL-1 for Cladosporium sp. ECGF19 to 12.68 mg mL-1 for Rhizomucor sp. ECGF18. In the sequence, Penicillium sp. ECGF02 and Rhizomucor sp. ECGF18, were previously select and, further evaluated in solid-state fermentation. Results confirmed the high extracellular lipase-activity of Penicillium sp. ECG02 and the high intracellular lipase activity of Rhizomucor sp. ECG18. Rhizomucor sp. ECG18 showed potential for use in future research, in the form of whole-cell lipases, wastewater treatment, and as a biocatalyst in the production of biodiesel from oil residues.
机译:真菌生物质作为脂肪酶生物催化剂的使用代表了一种有吸引力的方法,用于处理含油废水以及由油和残留油脂生产生物柴油,因为它具有更高的稳定性,可重复使用的可能性和较低的成本。在这项工作中,从巴西圣埃斯皮里图联邦大学一家餐馆的油脂捕集器浮渣中分离出20种丝状真菌。真菌包括那些属于曲霉属,白僵菌属,葡萄孢属,枝孢菌属,炭疽菌属,镰刀菌属,土生真菌属,青霉菌,根瘤菌属和黄萎病菌属的真菌。定量真菌脂肪酶活性和生物量产生。脂肪酶活性范围为Rhizomucor sp。的0.13 U mg-1蛋白。 ECGF18至Penicillium sp。的18.06 U mg-1蛋白。 ECGF02,Cladosporium sp。的生物量产生范围为7.61 mg mL-1。对于根瘤菌(Rhizomucor sp。)ECGF19至12.68 mg mL-1。 ECGF18。按照顺序,青霉菌sp。 ECGF02和Rhizomucor sp。预先选择了ECGF18,并在固态发酵中进行了进一步评估。结果证实了青霉菌的高细胞外脂肪酶活性。 ECG02和Rhizomucor sp。的高细胞内脂肪酶活性。心电图18。根瘤菌ECG18表现出了以全细胞脂肪酶,废水处理以及从油渣中生产生物柴油的生物催化剂形式用于未来研究的潜力。

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