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In vitro regenerative potentials of the medicinal plant Abutilon indicum (L.) Sweet

机译：药用植物Abutilon indicum（L.）Sweet的体外再生潜力

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摘要

The present study was undertaken to evaluate the most suitable concentration of growth regulators for callus induction and subsequent organogenesis in Abutilon indium (L.) Sweet (Malvaceae) leaf, stem and seed explants. Since the plant has a lot of medicinal importance, it was subjected to culture in vitro. Kinetin for seeds, 2,4-dichlorophenoxyacetic acid (2,4-D), benzyl amino purine (BAP), naphthalene acetic acid (NAA) and indole-3-acetic acid (IAA) for leaf as well as BAP and? kinetin for stem were the growth regulators used at different concentrations individually. Results showed that growth regulators at different combinations on subculture resulted in the regeneration of shoots. The excised shoots were transferred to liquid medium for in vitro rhizogenesis and plantlets were acclimatized in the laboratory conditions before they were transferred to garden soil. Histologically, callus sections were with active centres, embryoids and multiple shoot buds. Cytological variations such as multinucleate, chromosome bridges, polyploidy and tracheary elements were noticed in callus cells. The regenerated plants were morphologically and cytologically similar to the in vivo plants.
机译：进行本研究以评估在Ab麻（甜）（Ma科）叶，茎和种子外植体中愈伤组织诱导和随后器官发生的最合适浓度的生长调节剂。由于该植物具有许多医学重要性，因此对其进行了体外培养。种子用动蛋白，叶子用2，4-二氯苯氧基乙酸（2,4-D），苄基氨基嘌呤（BAP），萘乙酸（NAA）和吲哚-3-乙酸（IAA）以及茎的激动素是分别以不同浓度使用的生长调节剂。结果表明，继代培养中不同组合的生长调节剂导致了芽的再生。将切下的芽转移到液体培养基中以进行体外生根，并在将苗移入花园土壤之前使其在实验室条件下适应环境。组织学上，愈伤组织切片具有活跃的中心，胚状体和多个芽。在愈伤组织细胞中发现了细胞学变异，如多核，染色体桥，多倍体和气管元件。再生的植物在形态和细胞学上与体内植物相似。

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