• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >High genetic variation of Portunus pelagicus from Makassar Straits revealed by RAPD markers and mitochondrial 16S rRNA sequences
【24h】

High genetic variation of Portunus pelagicus from Makassar Straits revealed by RAPD markers and mitochondrial 16S rRNA sequences

机译：RAPD标记和线粒体16S rRNA序列揭示了望加锡海域梭子蟹的高遗传变异

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The blue swimmer crab, Portunus pelagicus, is presently managed as a single stock. In this study, random amplified polymorphic DNA (RAPD) markers and 16S rRNA sequences were used to understand the genetic diversity and parentage of P. pelagicus from Makassar Strait. A total of 150 samples were collected from the sea, grouped by gender and morphological features. DNA genome was performed at approximately 23130 base pairs (bp) and mitochondrial 16S rRNA sequences in the 500 to 600 bp. The percentage of polymorphic band using OPA-5, OPA-11, and OPA-17 primers was high (44.4 to 100%). Genetic identity ranged between 0.7266 and 0.9050 and genetic distance between 0.1008 and 0.3214. Alignment of 16S rRNA sequences shows 96 to 99% homology with P. pelagicus available in GenBank. Both RAPD markers and 16S rRNA sequences indicate that there was a high genetic variation observed among population, which formed two clusters. Specific unique bands found at 450 and 600 bp in OPA-11 gives an indication of hybridization among the population. The variation of white spot pattern on the carapace can be used as indicators of differentiation in population and parentage of P. pelagicus. Therefore, farmers or hatchery operators can continue to use the population as sources of natural broodstock.
机译：蓝色游泳蟹，Portunus pelagicus，目前作为单一种群进行管理。在这项研究中，随机扩增的多态性DNA（RAPD）标记和16S rRNA序列用于了解望加锡海峡海域P. pelagicus的遗传多样性和亲本。根据性别和形态特征，从海中总共收集了150个样本。 DNA基因组以大约23130个碱基对（bp）和500至600 bp的线粒体16S rRNA序列进行。使用OPA-5，OPA-11和OPA-17引物的多态条带百分比很高（44.4％至100％）。遗传同一性介于0.7266和0.9050之间，遗传距离介于0.1008和0.3214之间。 16S rRNA序列的比对显示与GenBank中可获得的P. Pelagicus有96％至99％的同源性。 RAPD标记和16S rRNA序列均表明在人群中观察到高遗传变异，形成了两个簇。在OPA-11中发现450和600 bp处的特定独特条带表明了群体之间的杂交。甲壳上的白斑图案的变化可以用作区分P. Pelagicus的种群和亲缘关系的指标。因此，养殖者或孵化场经营者可以继续利用这些种群作为自然亲鱼的来源。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号