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首页> 外文期刊>African Journal of Biotechnology >Production of laccase without inducer by Chaetomium species isolated from Chettaba forest situated in the East of Algeria
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Production of laccase without inducer by Chaetomium species isolated from Chettaba forest situated in the East of Algeria

机译：从位于阿尔及利亚东部的切塔巴森林中分离到的毛壳菌属物种生产无诱导剂的漆酶

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摘要

A positive laccase strain which showed a positive reaction with gua?acol was isolated from Chettaba Forest, which is situated in Constantine, the East of Algeria. It was identified as Chaetomium species (Ref 051A) according to the morphological and ribosomal internal transcribed spacer (ITS) DNA genomic sequence analysis. Laccase activity was determined by using 2,2-azinobis(3- ethylbenzthiazoline-6-sulfonate) diammonium salt (ABTS) as a substrate. Its highest activity was achieved by using potato dextrose broth (PDB) as a culture medium. Metal ion CuSO4 had no positive effect on laccase production. The laccase activity obtained in submerged fermentation (20 L) was higher than that produced in Erlenmeyer flask (500 ml). The study of pH and temperature effects showed that pH optimum was 5 and 6, and temperature was 35°C. Laccase produced by Chaetomium spp. can be used in industrial production.
机译：从位于阿尔及利亚东部康斯坦丁的切达巴森林中分离出一种阳性漆酶菌株，该菌株显示出与愈创木酚的阳性反应。根据形态和核糖体内部转录间隔区（ITS）DNA基因组序列分析，它被鉴定为Chaetomium物种（Ref 051A）。漆酶活性是通过使用2,2-叠氮基双（3-乙基苯并噻唑啉-6-磺酸盐）二铵盐（ABTS）作为底物来确定的。它的最高活性是通过使用马铃薯右旋糖肉汤（PDB）作为培养基来实现的。金属离子CuSO4对漆酶的生产没有积极影响。浸入式发酵（20 L）中获得的漆酶活性高于锥形瓶（500 ml）中产生的漆酶活性。对pH和温度影响的研究表明，最适pH为5和6，温度为35°C。 Chaetomium spp生产的漆酶。可用于工业生产。

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