• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Potentiality of lytic bacteriophages and their virolysins in lysing multi-drug resistant Salmonella typhi
【24h】

Potentiality of lytic bacteriophages and their virolysins in lysing multi-drug resistant Salmonella typhi

机译：溶解性噬菌体及其溶菌素在溶解多重耐药性伤寒沙门氏菌中的潜力

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacteriophage virolysins or lytic enzymes are bacterial peptidoglycan hydrolases responsible for lysing bacterial cells. Consequently, they are used as enzybiotics alongside with bacteriophage therapy to remedy multi-drug resistant Salmonella typhi. The objective of this study was to evaluate the potentiality of lytic bacteriophages and their virolysins in curing multi-drug resistant S. typhi. S. typhi was isolated and identified according to WHO and ISO guidelines. Antibiotics susceptibilities were tested using CLSI recommendations. Correspondingly, bacteriophage-lysing efficiency was assayed by plaques formation using the double-layer agar technique. Virolysins were extracted using ultracentrifugation and purified by dialysis after buffering in ammonium sulfate. Virolysins activity was determined by measuring the reaction mixtures spectrophotometrically (bacteria incubated as substrate in 37°C for 4 h). The phages and virolysins kinetics exponential rates were calculated using specific differential equations. Susceptibility data plotted based on antibiogram criteria confirmed that 33% of S. typhi isolates were multi-drug resistant. For bacteriophage replication and multiplicity of infection, phages were amplified to produce the maximum particles of titers. The phage titration data fit on sonogram revealed exponential decay of S. typhi incubated for 12 h. Meanwhile, the enzyme kinetics exponential decay on double reciprocal plot showed irretrievable relationship of host decay in 4 h. Since phages depend on their lytic cycle in lysing bacterial host, their enzymes have more capability in decaying the host; besides they are safe and time-saving when used in the treatment of antibiotics resistant S. typhi.
机译：噬菌体病毒溶素或裂解酶是负责裂解细菌细胞的细菌肽聚糖水解酶。因此，它们与细菌噬菌体疗法一起被用作酶制剂，以治疗多重耐药性伤寒沙门氏菌。这项研究的目的是评估溶解性噬菌体及其溶菌素在治疗多重耐药性伤寒沙门氏菌中的潜力。伤寒沙门氏菌是根据WHO和ISO指南进行分离和鉴定的。使用CLSI建议测试了抗生素敏感性。相应地，使用双层琼脂技术通过噬斑形成来测定噬菌体的裂解效率。使用超速离心提取溶溶素，并在硫酸铵中缓冲后通过透析纯化。通过分光光度法测定细菌混合物的溶菌素活性（细菌作为底物在37°C下孵育4小时）。使用特定的微分方程计算噬菌体和溶菌素动力学指数速率。根据抗菌素标准绘制的药敏性数据证实，33％的伤寒沙门氏菌具有多重耐药性。对于噬菌体复制和感染多重性，扩增噬菌体以产生最大滴度的颗粒。超声图上的噬菌体滴定数据拟合显示伤寒沙门氏菌温育12小时后呈指数衰减。同时，在双倒数图上的酶动力学指数衰减显示了在4小时内宿主衰减的不可逆的关系。由于噬菌体取决于其裂解细菌宿主的裂解周期，因此它们的酶具有更强的降解宿主能力。此外，当用于治疗耐药性伤寒沙门氏菌时，它们是安全且省时的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号