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首页> 外文期刊>African Journal of Biotechnology >Synthesis of silver nanoparticles using wild Cucumis anguria: Characterization and antibacterial activity
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Synthesis of silver nanoparticles using wild Cucumis anguria: Characterization and antibacterial activity

机译：使用野生黄瓜无头刺的银纳米粒子的合成：表征和抗菌活性。

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In the present study silver nanoparticles were synthesized using wild Cucumis anguria leaf extract using the green route which is a simple, cost-effective and an environmentally friendly technique. When the aqueous silver ions were exposed to the wild C. anguria leaf extract, they were reduced and this resulted in the formation of silver nanoparticles. The conditions of synthesis were optimized by adjusting the pH, time and amount of leaf extract. The synthesized silver nanoparticles were characterized by different techniques, namely ultra-violet visible spectroscopy, Fourier Transform Infrared Spectroscopy, X-ray diffraction and transmission electron microscopy. The ultra violet-visible spectrum of the synthesized silver nanoparticles shows a maximum peak at 420 nm. Fourier transform infrared spectroscopy results show the presence of alcohols, aromatics and amines suggesting the presence and binding of proteins with silver nanoparticles. Transmission electron microscopy analysis shows that the silver nanoparticles synthesized were spherical in shape with their sizes ranging between 11 and 27 nm depending on the pH conditions. X-ray diffraction analysis results show the presence of silver metal nanoparticles and their crystalline nature. The antibacterial activity of the silver nanoparticles synthesized was evaluated against Staphylococcus aureus (Gram-positive) and Escherichia coli (Gram-negative) bacterial and kanamycin was used as a control. The green chemistry route has proven that silver nanoparticles can be synthesized using C. anguria leaf extract in which biomolecules effectively act as reducing and capping agents.
机译：在本研究中，使用绿色路线的野生黄瓜角叶提取物合成了银纳米颗粒，这是一种简单，经济高效且环保的技术。当含水银离子暴露于野生的C. anguria叶提取物时，它们被还原，这导致形成了银纳米颗粒。通过调节pH，时间和叶提取物的量来优化合成条件。合成的银纳米颗粒通过紫外可见光谱，傅立叶变换红外光谱，X射线衍射和透射电子显微镜等不同技术进行表征。合成的银纳米颗粒的紫外-可见光谱在420nm处显示最大峰。傅里叶变换红外光谱结果表明存在醇，芳族化合物和胺，这表明蛋白质与银纳米颗粒的存在和结合。透射电子显微镜分析表明，所合成的银纳米颗粒为球形，其大小在11至27 nm之间，具体取决于pH条件。 X射线衍射分析结果表明银金属纳米颗粒的存在及其结晶性质。评价了合成的银纳米颗粒对金黄色葡萄球菌（革兰氏阳性）和大肠杆菌（革兰氏阴性）细菌的抗菌活性，并将卡那霉素用作对照。绿色化学路线已证明，可以使用安哥拉假单胞菌叶提取物合成银纳米颗粒，其中生物分子有效地充当还原剂和加帽剂。

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