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首页> 外文期刊>African Journal of Biotechnology >Deactivation of tannins in raisin stalk by polyethylene glycol-600: Effect on degradation and gas production in vitro
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Deactivation of tannins in raisin stalk by polyethylene glycol-600: Effect on degradation and gas production in vitro

机译：聚乙二醇600使葡萄干茎中的单宁酸失活：对体外降解和产气的影响

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An experiment was conducted to assess the effects of polyethylene glycol (PEG-6000) and urea on dry and organic matter digestibility (IVDMD and IVOMD, respectively)?and gas production?in vitro. Raisin stalk contained 8.6% crude protein, 85% dry matter, 7% ash, 13.95% total extractable phenol (TEPH) and 2.13% total extractable tannin (TET). The experimental treatments were: raisin with no supplementation as control (C); supplemented with 3% urea (U 3%), 5% urea (U 5%) or 3% urea plus 5% PEG (U-PEG) per DM. The rumen liquor fluid was obtained from two ruminal-cannulated fat-tailed sheep. TEPH and TET were determined?andin vitro?incubation was?also?conducted. The results indicated that the PEG increased IVOMD and IVDMD. The gas produced from time 0 to 3 and 3 to 6 h of incubation were significantly higher in PEG treatment than that of other groups (P?< 0.05).?The U-PEG?treatment increased?the?total gas production in 96 h, but it was not?statistically?significant (P?= 0.06).?Gas production in 96 h incubation using 300 mg fresh sample was significantly higher than 200 mg sample (P?< 0.05). It is concluded that the negative effect of tannin on DM and OM digestibility and also gas production of raisin stalk?in vitro?could be alleviated by PEG treatment.
机译：进行了一项实验，以评估聚乙二醇（PEG-6000）和尿素对体外干物质和有机物消化率（分别为IVDMD和IVOMD）和产气量的影响。葡萄干茎中含有8.6％的粗蛋白，85％的干物质，7％的灰分，13.95％的可萃取单宁（TEPH）和2.13％的可萃取单宁（TET）。实验处理是：不添加任何葡萄干作为对照（C）;每DM补充3％尿素（U 3％），5％尿素（U 5％）或3％尿素加5％PEG（U-PEG）。瘤胃液是从两只瘤胃插管的肥尾羊获得的。测定了TEPH和TET，并进行了体外培养。结果表明PEG增加了IVOMD和IVDMD。在PEG处理中，从孵化0到3和3到6 h产生的气体明显高于其他组（P <0.05）。U-PEG处理在96 h时增加了总气体的产生。 ，但无统计学意义（P = 0.06）。使用300 mg新鲜样品孵育96小时后的气体产量显着高于200 mg样品（P <0.05）。结论是，单宁对DM和OM消化率的不利影响以及葡萄干茎在体外的产气可以通过PEG处理得到缓解。

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