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Genetic analysis of Myanmar Vigna species in responses to salt stress at the seedling stage

机译:苗期盐胁迫下缅甸Vi豆的遗传分析

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Twelve (12)?Vigna?genotypes were investigated for the evaluation of their tolerance levels in responses to four concentrations of NaCl (0, 75, 150 and 225 mM) at seedling stage. In the investigation, salt stress inhibited almost all the growth parameters as well as relative water content; however, the degree of reduction was highly dependent on different genotypes and salinity levels. Generally, the control plants showed higher degree of all measured parameters than those of salt stress plants. ?Analysis of the heredity parameters based on the 12 investigated genotypes showed different genotypic variance of the salt tolerance index (STI) values. Salinity stress induced two new bands between 45 and 22 kDa, respectively, in salt tolerant genotypes. Furthermore, band intensity of the salt treated genotypes was higher than the control plants. Ward’s clustering technique was clearly divided into two clusters, A and B, according to their levels of salt tolerance. Considering their STI values of growth parameters, two genotypes V7 and V4 were identified as salt tolerant, whereas, V2, V6, V9, V8, V11 and V1 were recognized as salinity susceptible genotypes. These results suggest that, the genetically diverse accessions resistant to salt stresses within the?Vigna?genotypes can be of considerable practical value for studying the mechanism of salt tolerance and for the provision of genetic resources for salinity breeding program.
机译:研究了十二种(12)?Vigna?基因型,以评估它们在苗期对四种浓度的NaCl(0、75、150和225 mM)的耐受水平。在调查中,盐胁迫抑制了几乎所有的生长参数以及相对含水量。然而,减少的程度高度依赖于不同的基因型和盐度水平。通常,对照植物显示出比盐胁迫植物更高的所有测量参数度。 ?根据12种调查的基因型对遗传参数进行的分析显示,盐耐受指数(STI)值的基因型差异很大。盐胁迫在耐盐基因型中分别诱导了两个新的条带,分别位于45和22 kDa之间。此外,盐处理的基因型的条带强度高于对照植物。根据其耐盐性水平,沃德的聚类技术明显分为A和B两类。考虑到生长参数的STI值,两个基因型V7和V4被确定为耐盐的,而V2,V6,V9,V8,V11和V1被视为盐分敏感的基因型。这些结果表明,在“ Vigna”基因型内抗盐胁迫的遗传多样性种质对于研究耐盐性机制和为盐分育种计划提供遗传资源具有重要的实用价值。

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