• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>African Journal of Biotechnology >Isolation and detection of taxol, an anticancer drug produced from Lasiodiplodia theobromae, an endophytic fungus of the medicinal plant Morinda citrifolia
【24h】

Isolation and detection of taxol, an anticancer drug produced from Lasiodiplodia theobromae, an endophytic fungus of the medicinal plant Morinda citrifolia

机译：紫杉醇的分离和检测，紫杉醇是一种从药用植物巴戟天的内生真菌中生来的拉索氏杆菌产生的抗癌药

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

To determine the production of taxol from an endophytic fungus,?Lasiodiplodia theobromae?isolated from the medicinal plant?Morinda citrifolia?and also, to evaluate its cytotoxicity against human breast cancer cell line, taxol produced by the test fungus in MID culture medium was isolated for its characterization. The presence of taxol was confirmed by different chromatographic and spectroscopic analyses. The quantity of taxol produced by the fungus was calculated and estimated to be 245 μg/l. The fungal taxol was tested for its bioactivity against human cancer cell line (MCF-7) and the results showed that, the taxol possessed anticancer activity. The production of taxol was achieved from an endophytic fungus,?L. theobromae. The screened taxol showed a potential toxicity against breast cancer cell lines. Fungal based production of taxol from an endophytic fungus would be the most desirable and alternate source of supply. This study proved that the fungal endophyte?L. theobromae?is an excellent candidate for an alternate source of taxol supply. Confirmations of the?in vitro?activity of taxol against human breast cancer cell lines should encourage further research. Furthermore, it is the first report of the screening of taxol from the fungus?L. theobromae, isolated from the medicinal plantM. citrifolia.
机译：为了确定内生真菌产生紫杉醇的方法，从药用植物吗啉单胞菌中分离出了拟南芥（Lasiodiplodia theobromae），并评估其对人乳腺癌细胞系的细胞毒性，在MID培养基中分离了由测试真菌产生的紫杉醇。为其表征。通过不同的色谱和光谱分析证实了紫杉醇的存在。计算出由真菌产生的紫杉醇的量，估计为245μg/ l。测试了真菌紫杉醇对人癌细胞系（MCF-7）的生物活性，结果表明，紫杉醇具有抗癌活性。紫杉醇的产生是通过内生真菌ΔL实现的。 ob科。筛选的紫杉醇显示出对乳腺癌细胞系的潜在毒性。从内生真菌的基于真菌的紫杉醇生产将是最理想的替代供应来源。这项研究证明了真菌内生菌？ theobromae？是紫杉醇替代来源的极佳候选者。紫杉醇对人乳腺癌细胞系的“体外”活性的证实应鼓励进一步的研究。此外，这是首次从真菌中筛选紫杉醇的报道。从药用植物中分离出来的科。桔梗。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号