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首页> 外文期刊>African Journal of Biotechnology >Production and verification of heterozygous clones in Japanese flounder, Paralichthys olivaceus by microsatellite marker
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Production and verification of heterozygous clones in Japanese flounder, Paralichthys olivaceus by microsatellite marker

机译：利用微卫星标记技术在日本比目鱼Oliparaeus olivaceus中生产和验证杂合子

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Mitotic gynogenetic diploids Japanese flounder?Paralichthys olivaceus?were produced by activating eggs with ultra violet (UV) irradiated sperm of red sea bream (Pagrus major), followed by hydrostatic pressure treatment to block the first mitotic division. By crossing two mitotic gynogenetic diploid females with two males, two heterozygous clones of Japanese flounder were produced. Microsatellite marker was used to confirm the genetic status of maternal parents and their progenies.?20polymorphic microsatellite markers were chosen, covering 16 out of the total 24 linkage groups. The four maternal parents used in this experiment were completely heterozygous, while four mitotic gynogenetic diploids were homozygous for each marker. The genotypes of heterozygous clone progenies were identical and the combination of parental alleles, demonstrates the successful development of cloning.
机译：有丝分裂的雌雄同体二倍体日本比目鱼是通过用紫外线（UV）照射的红鲷（Pagrus major）精子激活卵，然后进行静水压力处理来阻止第一个有丝分裂分裂而产生的。通过将两个有丝分裂的雌核发育二倍体雌性与两个雄性杂交，产生了两个日本比目鱼杂合子克隆。微卫星标记被用于确认母亲父母及其后代的遗传状况。选择了20个多态微卫星标记，覆盖了全部24个连锁组中的16个。在该实验中使用的四个母体父母是完全杂合的，而每个标记的四个有丝分裂的雌核发育二倍体是纯合的。杂合子代后代的基因型相同，并且亲本等位基因的组合表明克隆的成功发展。

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