Identification of bacterial strains in viili by molecular taxonomy and their synergistic effects on milk curd and exopolysaccharides production

Tingtao Chen; Qianglai Tan; Mengjuan Wang; Shunqiang Xiong; Shuying Jiang Qinglong Wu; Shengjie Li; Cheng Luo; Hua Wei

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Identification of bacterial strains in viili by molecular taxonomy and their synergistic effects on milk curd and exopolysaccharides production

机译：通过分子分类学鉴定小肠小球菌中的细菌菌株及其对豆腐和胞外多糖产生的协同作用

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Viili, also known as viilia, is a traditional fermented dairy product, which is popular in Finland. However, little research has been conducted on organisms’ isolation and their interaction during milk fermentation. In this study, culture-dependent and independent methods had been used to identify isolates. Also, their synergistic effects on milk curd and exopolysaccharides (EPSs) yield?was?evaluated. WhenLactobacillus plantarum,?Streptococcus thermophilus,?Lactobacillus paracasei,Bacillus cereus?and?Lactobacillus delbrueckii?were successfully separated and screened from viili by using the combination of conventional microbiological cultivation with polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE), they were individually inoculated into sterilized skimmed milk or combined as co-cultures to determine the curd time and the yield of EPSs. Results show?that?L. delbrueckii?presented the strongest curding capability at 5 h and combination of?L. paracasei,?B. cereus?and?L. plantarum?brought the highest yields of EPSs (186.71 mg/L). Moreover, it suggested that the?synergistic effects among the isolates had shortened the curding time and increased the production of EPSs.

机译：Viili，也称为Viilia，是一种传统的发酵乳制品，在芬兰很流行。但是，关于牛奶发酵过程中生物的分离及其相互作用的研究很少。在这项研究中，依赖于文化的和独立的方法已被用来识别分离株。此外，还评估了它们对豆腐和胞外多糖（EPS）产量的协同作用。通过将常规微生物培养与聚合酶链反应-变性梯度凝胶电泳（PCR-DGGE）结合使用，成功地分离并从植物中分离出了植物乳杆菌，嗜热链球菌，副干酪乳杆菌，蜡状芽孢杆菌和德氏乳杆菌。将它们分别接种到灭菌的脱脂牛奶中或合并在一起作为共培养物以确定凝乳时间和EPS的产量。结果表明？ delbrueckii？在5 h和？L的组合下表现出最强的凝结能力。 B.副干酪蜡膜和？车前草的EPS产量最高（186.71 mg / L）。此外，这表明分离株之间的协同效应缩短了凝乳时间并增加了EPS的产生。

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《African Journal of Biotechnology》 |2011年第74期|共7页
作者
Tingtao Chen; Qianglai Tan; Mengjuan Wang; Shunqiang Xiong; Shuying Jiang Qinglong Wu; Shengjie Li; Cheng Luo; Hua Wei;
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中图分类生物工程学（生物技术）;
关键词
ViiliPCR-DGGEexopolysaccharideslactic acid bacteria;

机译：ViiliPCR-DGGE胞外多糖乳酸菌;

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专利

1. ROLE OF THE PROPORTION OF YOGHURT BACTERIAL STRAINS IN MILK SOURING AND THE FORMATION OF CURD QUALITATIVE CHARACTERISTICS [J] . DOROTA CAIS-SOKOLINSKA, MIROSLAW M. MICHALSKI, JAN PIKUL Bulletin of the Veterinary Institute in Pulawy . 2004,第4期

机译：酸奶细菌菌比例在乳汁中的作用和凝乳定性特征的形成。
2. A taxonomic study to establish the relationship between exopolysaccharide-producing bacterial strains living in diverse hypersaline habitats [J] . Martinez-Canovas MJ, Quesada E, Martinez-Checa F, Current Microbiology: An International Journal . 2004,第5期

机译：建立分类研究，以建立生活在不同高盐生境中的产胞外多糖的细菌菌株之间的关系
3. MICROBIAL QUALITY AND MOLECULAR IDENTIFICATION OF PATHOGENIC BACTERIAL STRAINS COLLECTED FROM RAW CAMEL'S MILK IN TAIF REGION [J] . Samy Mahmoud F., Yasser El-Halmouch, Othman A. L. Zhrani M., Journal of Camel Practice and Research . 2017,第1期

机译：陶克牛奶中原料骆驼牛奶中致病菌菌株的微生物质量和分子鉴定
4. Exopolysaccharide-producing lactic acid bacterium strains as functional starter cultures in the production of fermented milk [C] . L. De Vuyst, M. Zamfir, B. Degeest, International Dairy Federation Seminar on Aroma and Texture of Fermented Milk . 2003

机译：在发酵乳的生产中，将外偶糖产生乳酸菌作为功能性起动培养物
5. Identification of a type III secretion system in Lysobacter enzymogenes strain C3: Molecular characterization and functional analysis of the pathway as an integral part in bacterial-fungal interactions. [D] . Reedy, Ralph Michael. 2004

机译：鉴定溶菌酶C3菌株中的III型分泌系统：该分子作为细菌-真菌相互作用不可或缺的部分的分子表征和功能分析。
6. Isolation and characterization of exopolysaccharide‐producing strains of Lactobacillus bulgaricus from curd [O] . Khubaib Ali, Muhammad Huzaifa Mehmood, Muhammad Ahmad Iqbal, 2019

机译：凝乳中保加利亚乳杆菌产外多糖菌株的分离与鉴定
7. The bacterial identification and antimicrobial susceptibility testing are usually performed manually. Recently, the number of samples has increased and the need for automatification has grown. BioMerieux has investigated Vitek2 for this purpose. HUSLAB has assigned us to compare the identification and antimicrobial results with respect to routine methods and Vitek2. Furthermore, we evaluate test reactions, ESBL (Extended Spectrum Beta-Lactamase) production and the time what it takes to get results with Vitek2. In this study, we examined a total of 183 bacterial isolates including 76 ESBL-strains, 40 nonfermenting rods, 41 fastidious bacteria and 26 fresh clinical isolates. The results of routine methods were gathered from the patient database and following this, compared to the results given by Vitek2. Vitek2 correctly identified more than 96 f the ESBL-strains and fresh clinical isolates. The results of nonfermenting rods and fastidious bacteri a were 70 oncordant with those of the routine methods. Test reaction results of Api20E were 94 nd Api20NE 70 n concordance with Vitek2 results. Approximately 80 f all Vitek2 antimicrobial results and 90 f ESBL test results were correct. Compared to earlier studies, Vitek2 performed the tests faster than the routine methods. These results suggest that Vitek2 is capable of giving rapid and accurate results. However, nonfermenting rods and fastidious bacteria should be studied further. [O] . Järveläinen Marika, Roslund Iira, Silander Maarit 2006

机译：细菌鉴定和抗菌药敏试验通常是手动进行的。最近，样品的数量增加了，并且对自动化的需求也在增长。 BioMerieux为此进行了Vitek2的研究。 HUSLAB已指派我们比较常规方法和Vitek2的鉴定和抗菌结果。此外，我们还评估了测试反应，ESBL（扩展频谱β-内酰胺酶）的产生以及使用Vitek2获得结果所需的时间。在这项研究中，我们检查了总共183个细菌分离株，包括76个ESBL菌株，40个非发酵棒，41个营养细菌和26个新鲜临床分离株。从患者数据库中收集常规方法的结果，然后将其与Vitek2给出的结果进行比较。 Vitek2正确鉴定出超过96种ESBL菌株和新鲜的临床分离株。与常规方法相比，非发酵棒和精制细菌的结果为70。 Api20E的测试反应结果与Vitek2结果一致为94nd Api20NE 70 n。所有Vitek2抗菌结果约80 f和ESBL测试结果90 f正确。与早期的研究相比，Vitek2的测试速度快于常规方法。这些结果表明Vitek2能够给出快速而准确的结果。但是，应进一步研究非发酵棒和营养细菌。

Identification of bacterial strains in viili by molecular taxonomy and their synergistic effects on milk curd and exopolysaccharides production

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