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首页> 外文期刊>African Journal of Biotechnology >In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis
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In vitro plant regeneration in rough lemon (Citrus jambhiri Lush) by direct organogenesis

机译：直接器官发生在粗柠檬（Citrus jambhiri Lush）中进行体外植物再生

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This?study?was aimed to optimize the organogenesis in rough lemon (Citrus jambhiri?Lush), an important citrus rootstock of India. Organogenesis was induced in epicotyl segments of rough lemon seedlings. Three important factors influencing organogenesis?in vitro?viz. hormone combination, cut modes and photoperiod were evaluated. Etiolation of seedlings had a positive effect on bud formation and higher number of buds per explant was obtained from etiolated seedlings. Among the cut modes, transverse cut performed better for its effect on the number of regenerated buds and shoots per explant. The best response regarding the number of adventitious buds formed per explant was observed with BAP at the concentration of 2.0 mg/l along with malt extract (500 ppm) and additional dose of 25 g/l sucrose.
机译：这项研究旨在优化粗柠檬（Citrus jambhiri？Lush）（印度重要的柑橘砧木）的器官发生。在粗糙的柠檬幼苗的上胚轴节段中诱导器官发生。影响器官发生的三个重要因素是体外。评估激素组合，切割方式和光周期。苗化对幼苗的芽形成有积极作用，并且从黄化的幼苗中获得更高的单植株芽数。在切割模式中，横向切割对每个外植体再生芽和新芽的数量影响较大。对于浓度为2.0 mg / l的BAP以及麦芽提取物（500 ppm）和25 g / l蔗糖的额外剂量，观察到关于每个外植体形成的不定芽数量的最佳响应。

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