Genetic variation in a population of Bipolaris oryzae based on RAPD-PCR in north of Iran

Mohammad Reza Safari Motlagh; Maesomeh Anvari

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Genetic variation in a population of Bipolaris oryzae based on RAPD-PCR in north of Iran

机译：基于RAPD-PCR的伊朗北部双子叶稻种群遗传变异

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Isolates of?Bipolaris oryzae?were analysed by RAPD techniques to determine the amount of intraspecific genetic variability. In order to do RAPD-PCR, seven primers were applied. At first, DNA of all isolates was isolated, and then DNA was amplified in thermocycler by using seven primers at a thermal program. As the result, segments of DNA by the size of 200 - 300 bp were produced. Cluster analysis using UPGMA method gave three groups. Levels of polymorphism were observed between DNA of different isolates. The pattern of RAPD bands could not show the direct correlation between polymorphism and climates or geographical areas.

机译：用RAPD技术分析米曲霉的分离物，以确定种内遗传变异量。为了进行RAPD-PCR，应用了七个引物。首先，分离所有分离物的DNA，然后在热循环仪中使用7个引物在热循环仪中扩增DNA。结果，产生了200-300bp大小的DNA片段。使用UPGMA方法进行的聚类分析分为三类。在不同分离株的DNA之间观察到多态性水平。 RAPD带的模式不能显示多态性与气候或地理区域之间的直接相关性。

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《African Journal of Biotechnology》 |2010年第36期|共5页
作者
Mohammad Reza Safari Motlagh; Maesomeh Anvari;
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中图分类生物工程学（生物技术）;
关键词
Bipolaris oryzaericegenetic variationRAPD;

机译：米氏双极生代遗传变异;

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Genetic variation in a population of Bipolaris oryzae based on RAPD-PCR in north of Iran

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