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首页> 外文期刊>African Journal of Biotechnology >Differential expression of ozone-induced gene during exposures to salt stress in Polygonum sibiricum Laxm leaves, stem and underground stem
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Differential expression of ozone-induced gene during exposures to salt stress in Polygonum sibiricum Laxm leaves, stem and underground stem

机译：盐胁迫下何首乌叶片，茎和地下茎中臭氧诱导基因的差异表达

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摘要

The response of plants to environmental stresses is characterized by a number of physiological and biochemical changes that ultimately result from the selective increase or decrease in the biosynthesis of a large number of distinct proteins. In this report, we describe the characterization of an ozone-induced transcript,?PcOZI-1, which has been identified from?Polygonum sibiricum?Laxm?by the rapid amplification of cDNA ends method (RACE).?PcOZI-1?mRNA in untreated plants was detected at low levels in underground stem, leaves and at higher levels in stem.?PcOZI-1?mRNA accumulation was transiently induced in stem 7-fold within the first 8 h of 3% NaHCO3?treatment.?PcOZI-1?mRNA accumulation was also induced?22-fold in underground stem after 72 h. Sequence analysis of?PcOZI-1revealed that it encodes a 8.45 kDa basic protein that contains a putative signal peptide. Our results suggest that PcOZI-1 represents a novel stress-related protein that accumulates in response to the production of active oxygen species.
机译：植物对环境胁迫的响应的特征在于许多生理和生化变化，这些变化最终归因于大量不同蛋白质的生物合成的选择性增加或减少。在本报告中，我们描述了臭氧诱导的转录本？PcOZI-1的特征，该转录本已通过快速扩增cDNA末端方法（RACE）从？Polygonum sibiricum？Laxm？中鉴定出来。？PcOZI-1？mRNA未经处理的植物在地下茎，叶中的含量低，而茎中的含量高。在3％NaHCO3处理的最初8小时内，茎中瞬时诱导了？PcOZI-1？mRNA的积累。PcOZI-1。 72小时后，地下茎中的mRNA积累也被诱导了22倍。对PcOZI-1的序列分析表明它编码了一个8.45kDa的碱性蛋白质，该蛋白质含有一个推定的信号肽。我们的结果表明，PcOZI-1代表一种新型的应激相关蛋白，可响应活性氧的产生而积累。

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