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首页> 外文期刊>African Journal of Biotechnology >In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant
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In vitro propagation of Rosa hybrida L. cv. Al-Taif Rose plant

机译：杂交蔷薇的体外繁殖Al-Taif玫瑰厂

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摘要

In this study, a protocol for?in vitro?propagation of?Rosa hybrida?L. cv. Al-Taif Rose was established using nodal segments harboring axillary buds as explants.?In vitro?stages of shoot initiation, multiplication and elongation were performed. Explants were cultured on solid Murashige and Skoog medium (MS) supplemented with different concentrations of benzyl aminopurine (BAP, 1, 2 and 3 mg/l) in combination with 1 mg/l kinetin (Kn). Effect of different concentrations and combinations of indole-3-acetic acid (IAA) and indole-3-butyric acid (IBA) on root formation of shoots were studied. The highest percentage of shoot initiation (85%) was observed on MS medium containing 2 mg/l BAP + 1 mg/l Kn, whereas maximum average number of multiplied shoots (2.7)was produced on?MS?medium with 3 mg/l?BAP?+ 1?mg/l Kn. Highest?average?number of elongated shoots?(26.7) was noticed on MS medium containing 1 mg/l BAP and 1 mg/l Kn. For rooting, highest percentage (66.7%) of rooted shoots was obtained on MS medium supplemented with 2 mg/l IBA. Plantlets with 4 to 5 roots of 3 to 5 cm length were successfully transferred to pots containing sterile peat moss for acclimatization.
机译：在这项研究中，用于“罗莎杂交种”的体外繁殖的方案。简历。 Al-Taif Rose是用带有腋芽的结节段作为外植体建立的。在体外，进行了芽的萌芽，繁殖和伸长。外植体在固体Murashige和Skoog培养基（MS）上培养，该培养基补充了不同浓度的苄基氨基嘌呤（BAP，1、2和3 mg / l）和1 mg / l激动素（Kn）。研究了吲哚-3-乙酸（IAA）和吲哚-3-丁酸（IBA）的不同浓度和组合对苗根形成的影响。在含2 mg / l BAP + 1 mg / l Kn的MS培养基上观察到最高的芽萌芽率（85％），而在3 mg / l的？MS？培养基中产生的平均平均增生芽数最多（2.7） ？BAP？+1？mg / l Kn。在含有1 mg / l BAP和1 mg / l Kn的MS培养基上，观察到最长的平均伸长芽数（26.7）。对于生根，在补充了2 mg / l IBA的MS培养基上可获得最高百分比（66.7％）的生根芽。将4至5根长3至5厘米长的幼苗成功转移到装有无菌泥炭藓的盆中进行驯化。

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